Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Activity Kits > TAF1L (BD2) Inhibitor Screening Assay Kit

TAF1L (BD2) Inhibitor Screening Assay Kit

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By Species
Human(7)
By Source
E. coli(5)
E.coli(2)
By Tag
GST(3)
His(2)
His & FLAG(2)
Cat.No.:
Kit-1876
Size:
384 reactions
Description:
The TAF1L (BD2) Inhibitor Screening Assay Kit is designed to measure the inhibition of TAF1L binding to its substrate. The key to the assay kit is specific binding of the TAF1L bromodomain 2 (a.a. 1517-1649) to the acetylated histone substrate. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing TAF1L and an inhibitor of choice is incubated with the biotinylated substrate for thirty minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.
Applications:
Useful for the study of bromodomain binding assays, screening bromodomain inhibitors and selectivity profiling.
Storage:
At least 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Kit Components:
GST-TAF1L (1517-1649): 80 µg; -80°CBET Bromodomain Ligand: 400 µl; -80°C Non-acetylated Ligand 1: 200 µl; -80°C 3x BRD Homogeneous Assay Buffer 2: 4 ml; -20°C3x BRD Homogeneous Detection Buffer 2: 3 ml; -20°C


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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