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Home > Products > Assay Kits > Activity Kits > TEV Protease Fluorometric Activity Assay Kit

TEV Protease Fluorometric Activity Assay Kit

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Product Overview:
TEV Protease Activity assay kit utilizes the ability of TEV Protease to cleave a synthetic Fluorescein-based peptide substrate to release fluorescein which can be easily quantified using a fluorometer or fluorescence microplate reader (Ex/Em = 490/560 nm). This assay kit is simple, rapid and can detect TEV Protease activity as low as 50 ng/well in viral lysates and purified protein samples.
100 assays
TEV Protease (EC:, Tobacco Etch Virus protease) is a cysteine protease that recognizes the cleavage site of Glu-Xaa- Xaa-YXaa-Gln-(Gly/Ser) and cleaves between Gln and Gly/Ser. The optimal sequence is Glu-Asn-Leu-Tyr-Phe-Gln-Ser/Glycine (ENLYFQS/G). TEV Protease has high specificity and great stability and is active over a wide range of temperatures (4-37°C) with an optimal activity at 34°C.
Detection of TEV Protease activity in viral lysates Determine activity of purified TEV Protease.
Target Species:
Viral lysates
Store kit at -20°C, protected from light. Briefly centrifuge small vials at low speed prior to opening. Read entire protocol before performing the assay.TEV Protease Assay Buffer: Add 100 µl of 1 M DTT to the TEV Protease Assay Buffer. Mix well. Store at -20°C. Bring to room temperature before use.TEV Protease (Positive Control): Aliquot and store at -80°C. Avoid repeated freeze/thaw.
Kit Components:
TEV Protease Assay Buffer: 25 mlTEV Protease Dilution Buffer: 10 ml1 M DTT: 100 μlTEV Protease (Positive Control): 10 μlTEV Protease Substrate: 100 μl5-FAM Standard (100 µM in DMSO): 100 μl
Detection method:
Fluorescence (Ex/Em = 490/560 nm)
Compatible Sample Types:
Viral Lysates, purified protein
Features & Benefits:
• Simple, rapid assay• Can detect as low as 50 ng/well of TEV Protease activity• Fluorometric Detection

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127

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