Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Activity Kits > TF (Human) Chromogenic Activity Assay Kit

TF (Human) Chromogenic Activity Assay Kit

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Cat.No.:
Kit-0813
Product Overview:
TF (Human) Chromogenic Activity Assay Kit is developed to determine human TF chromogenic activity in plasma, tissue, and cell culture supernatants.
Description:
The transmembrane protein Tissue factor (TF) is the physiologic trigger of coagulation in normal hemostasis. TF binds and allosterically activates factor VII (FVII). The TF-FVIIa complex cleaves factor IX and X, leading to thrombin generation. TF markedly enhances the ability of FVIIa to cleave both macromolecule and small peptidyl substrates. Inducible expression of TF in a variety of pathological conditions, including gram-negative sepsis and acute coronary syndromes, is associated with life-threatening thrombosis. In sepsis, TF expression within the vasculature leads to disseminated intravascular coagulation. TF also plays important roles in vasculogenesis, metastasis, and tumor-associated angiogenesis.
Target Species:
Human
Usage:
For research use only (RUO)
Storage:
Store components of the kit at 2-8°C or -20°C upon arrival up to the expiration date.Store Standard, Factor VII protein , Factor X protein, and FXa Substrate at -20°C.Store Microplate, Sample Diluent, and Assay Diluent at 2-8°C.Unused microplate wells may be returned to the foil pouch and resealed. May be stored for up to 30 days in a vacuum desiccator.Opened diluent may be stored for up to 30 days at 2-8°C.
Kit Components:
Microplate: A 96-well polystyrene microplate. 96(8x12) wellsSealing Tapes: Pressure-sensitive sealing tapes that can be cut to fit the format of the individual assay. 3 slicesSample Diluent 30 mLAssay Diluent 20 mLrhTF Standard (Lipoprotein): Recombinant human TF lipoprotein. 1 vialHuman FVII 1 vialHuman FX 1 vialFxa Substrate 2 vials


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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