|Description:||The Tankyrase-1 Histone Ribosylation Assay Kit (Antibody Detection) is designed to measure Tankyrase-1 activity for screening and profiling applications. The key to the Tankyrase-1 Histone Ribosylation Assay Kit (Antibody Detection) is a highly specific antibody that recognizes poly (ADP-ribose)-modified histone. After coating the plate with the histone mixture, only three simple steps are required. First, NAD+ is incubated with a sample containing assay buffer and enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.|
|Applications:||Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.|
|Storage:||12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.|
|Kit Components:||TNKS1: 2 µg; -80°C 5 x histone mixture: 1 ml; -80°CNAD+ : 250 µl; -80°C10x PARP Assay Buffer: 1 ml; -80°CPrimary antibody 11: 13 µl; -80°C Secondary HRP-labeled antibody 1: 10 µl; -80°CBlocking buffer: 80 ml; +4°C HRP chemiluminescent substrate A (2 components): 6 ml each; +4°C 8-well strip plate module: 1 plate (12 x 8-well strips); +4°C|
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