"TNKS1" Related Products

TNKS1 Histone Ribosylation Assay Kit (Antibody Detection) II

Cat.No.: Kit-1880
Size: 384 reactions
Description: The Tankyrase-1 Histone Ribosylation Assay Kit (Antibody Detection) is designed to measure Tankyrase-1 activity for screening and profiling applications. The key to the Tankyrase-1 Histone Ribosylation Assay Kit (Antibody Detection) is a highly specific antibody that recognizes poly (ADP-ribose)-modified histone. With this kit, only three simple steps on a microtiter plate are required for Tankyrase-1 reaction. First, NAD+ is incubated with a sample containing assay buffer and enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.
Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Storage: 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Kit Components: TNKS1: 4 µg; -80°C NAD+ : 500 µl; -80°C10x PARP Assay Buffer: 2 ml; -80°CPrimary antibody 11: 26 µl; -80°C Secondary HRP-labeled antibody 1: 20 µl; -80°CBlocking buffer: 160 ml; +4°C HRP chemiluminescent substrate A (2 components): 12 ml each; +4°C 384-well plate pre-coated with histone mixture: 1 plate; +4°C

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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