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Home > Products > Assay Kits > Activity Kits > Thioredoxin-1 assay Kit

Thioredoxin-1 assay Kit

"Trx-1" Related Products.
2x96 wells
The Thioredoxin-1 assay kit provides a simple, homogenous assay for screening modulators of Trx-1 enzymatic activity in microplates. This is accomplished by monitoring the Trx-1 catalyzed reduction of insulin in the presence of Dithiothreitol (DTT), resulting in the formation of insulin aggregates which then bind avidly to the red-emitting fluorogenic ProteoStat Trx-1 detection dye. The fluorescence-based assay provides a vastly improved assay signal window, improved lower detection limit, and superior Z"-score (>0.8) relative to turbidity measurements. Intra-plate and inter-plate CVs using this assay are typically 3-6%. Human recombinant thioredoxin-1 enzyme and the thioredoxin inhibitor, aurothiomalate, are included in the kit, as well as all reagents necessary for monitoring changes in Trx-1 activity. The ProteoStat Thioredoxin-1 assay kit is capable of providing a quantitative readout of Trx-1 enzymatic activity in a robust and high-throughput fashion and can be applied to identification of Trx-1 inhibitors from chemical libraries.
This kit provides a simple, homogenous assay for screening modulators of Thioredoxin-1 enzymatic activity.
Kit Components:
ProteoStat Trx-1 detection reagent: 20μLThioredoxin-1 (human) (recombinant): 2 vials x 80μLInsulin: 2 vials x 1.8μmolesAurothiomalate (inhibitor): 2 vials x 25nmolesPBE buffer: 25mlStop reagent: 1mlDTT: 2 vials x 1.3mlDeionized water: 5ml

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127

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