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Home > Products > Assay Kits > Activity Kits > Thrombin Activity Fluorometric Assay Kit

Thrombin Activity Fluorometric Assay Kit

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Product Overview:
Simple & convenient protocol.Can detect thrombin activity as low as.Kit contains all necessary reagents for measuring thrombin activity.
100 assays
Thrombin enzyme (Factor IIa, EC, a Serine protease, is an important clotting factor in the coagulation cascade that involves the conversion of soluble fibrinogen to insoluble active fibrin strands. In this pathway, Prothrombin is proteolytically converted into an active Thrombin. Thrombin is also a potent vasoconstrictor and mitogen implicated as a major factor in vasospasm following subarachnoid hemorrhage. Ruptured cerebral aneurysm blood clots around a cerebral artery releases thrombin, which in turn induces acute and prolonged narrowing of the blood vessel, potentially resulting in cerebral ischemia and infarction (stroke). In addition, it is a pro-inflammatory enzyme that may influence the onset and progression of atherosclerosis. Biovisions Thrombin activity assay kit utilizes the ability of Thrombin to proteolytically cleave a synthetic substrate and release a fluorophore, AMC, which can be easily quantified by fluorescence reader. This assay kit is simple, rapid and can detect Thrombin activity as low as 1 ng in samples.
Determine activity of pure Thrombin, Detect the activity of Thrombin in plasma
For Research Use Only! Not For Use in Humans.
Kit Components:
• Thrombin Dilution Buffer• Thrombin Assay Buffer• Thrombin Enzyme Standard • Thrombin Substrate
Detection method:
Fluorescence (Ex/Em = 350/450 nm)
Compatible Sample Types:

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

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