Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
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Zinc Assay Kit II

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Cat.No.:
Kit-0851
Product Overview:
Zinc Assay Kit is a quantitative colorimetric determination of zinc at 560 nm.
Description:
Zinc, a metallic chemical element, symbol Zn and atomic number 30 is chemically similar to Magnesium due to its similar size and sole oxidation state of +2. Zinc is an essential mineral of great biological significance since many enzymes require it as an essential cofactor. Examples of zinc"s biological roles include signal transduction, gene expression, regulation of apoptosis, synaptic plasticity and prostate gland function. Zinc Assay Kit is a convenient colorimetric assay in which Zinc binds to a ligand with development of absorbance at 560nm. It can be used with biological samples such as serum, plasma, csf or urine.
Target Species:
Mammals
Usage:
For research use only (RUO)
Storage:
Store the kit at +4°C and protect from light. Read the entire protocol before performing the assay. Synthetic rubber and glass can contain zinc which may leach into samples. For highest accuracy all glassware should be washed with dilute HCl, rinsed with distilled water and dried prior to use. Sample tubes such as Vacutainer and similar devices should be sealed with Parafilm rather than the butyl rubber stopper. Chelators such as EDTA will give artifactually low Zinc levels and should be avoided. Heparin, citrate and oxalate are acceptable anticoagulants. Most blood zinc (80%) is contained in erythrocytes and hemolysis will release large amounts into the serum. Abnormally high serum values obtained suggest the collection of another sample and retesting.
Kit Components:
Zinc Reagent 1. Cap code: WM. 16 mlZinc Reagent 2. Cap code: amber. 4 ml7% TCA. Cap code: clear. 5 mlZinc Standard (50 mM). Cap code: yellow. 0.1 ml
Detection method:
Colorimetric
Compatible Sample Types:
Biological Fluid, Cell Lysate, Cerebrospinal Fluid, Culture Medium, Plasma, Serum, Tissue Lysate, Urine


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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