Aconitase Enzyme Activity Microplate Assay Kit
Cat.No. : | Kit-0044 |
Product Overview : | Aconitase Enzyme Activity Microplate Assay Kit is a simple, reproducible, and sensitive tool for assaying aconitase from tissue homogenates or cell lysates. Unlike other assays this is not a coupled reaction and therefore only aconitase activity is required and measured. Aconitase catalyzes an equilibrium between aconitate, cis-aconitate and iso-citrate. These reactions are monitored by measuring the increase in absorbance at 240 nm associated with the formation of the cis-aconitate which has an extinction coefficient of 2.2 OD/mM per well. Therefore the rate of cis-aconitate production is proportional to aconitase activity. |
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Applications : | Functional Studies |
Storage : | Store UV microplate at room temperature.Store all other components store at 4°C. |
Products Types
◆ Assay kits | ||
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Ask a questionThe main function of aconitase is to participate in the TCA cycle, which is responsible for the generation of energy in cells. Aconitase catalyzes the interconversion of citrate to isocitrate, which is a critical step in the TCA cycle.
Aconitase has a unique iron-sulfur cluster at the enzyme's active site, which is essential for its catalytic activity. The enzyme has two active sites, one of which is tightly bound to the iron-sulfur cluster.
Techniques such as enzyme assays, western blotting, and RNA sequencing can be used to study aconitase activity and expression. Additionally, imaging techniques such as confocal microscopy and immuno-electron microscopy can be used to visualize the subcellular localization of aconitase.
Mutations in the aconitase gene have been associated with a rare genetic disorder called infantile cerebellar-retinal degeneration, which affects the development of the cerebellum and retina. Additionally, aconitase activity has been shown to be altered in various diseases, including cancer, neurodegenerative disorders, and cardiovascular disease.
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