Aflibercept Bioassay Kit

• Cat.No.: Kit-1613
• Product Overview: Aflibercept Bioassay kits provide a functional, robust, highly sensitive, and easy-to-use cell-based assay to study aflibercept potency and neutralizing antibodies. The bioassay kits contain all the reagents needed for a complete assay including cells, detection reagents, cell plating reagent, positive control agonist, and assay plates. The pre-validated, frozen cells have been manufactured for single-use and are provided in a ready-to-assay format that saves time and adds convenience.

Specification

• Storage: HEK 293 KDR/KDR Bioassay Cells
  Cells are shipped on dry ice and should arrive in a frozen state. To ensure maximum cell viability, thaw the vials as soon as possible upon receipt.
  If continued storage of the frozen vials is necessary, store as follows:
  ƒ Short term (24 hours or less): Store vials at -80°C immediately upon arrival. (DO NOT store at -80°C for more than 24 hours).
  ƒ Long term (greater than 24 hours): Vials should ONLY be stored in the vapor phase of liquid nitrogen (LN2).
  Safety Warning: A face shield, gloves, and lab coat should be worn at all times when handling frozen vials. The manufacturer of the
  cryovial recommends storing the vials in the vapor phase above the LN2. Upon thawing, if LN2 is present in the cryovial, it rapidly
  converts back to its gas phase which can result in the explosion of the vial upon its removal from the LN2 tank.
  
  Bioassay Detection Kit
  Store at -20°C. Once thawed, the detection reagents can be kept at 4°C for up to 4 days. For longer storage (up to the expiration date listed in the kit certificate of analysis), the reagent should be aliquoted and stored at -20°C until needed. Avoid multiple freeze-thaw cycles.
  To make aliquots suitable for testing one assay plate each, 1mL of Detection Reagent 1 per aliquot can be dispensed and frozen down. 4mL of Detection Reagent 2 per aliquot can be dispensed and frozen down separately. Do not mix the two reagents during aliquoting.
  
  AssayComplete Cell Plating Reagent
  Once thawed, the Cell Plating Reagent can be stored at 4°C for up to 4 weeks. For longer storage (up to the expiration date listed in the kit certificate of analysis), the reagent should be aliquoted and stored at -20°C until needed. Avoid multiple freeze-thaw cycles. To make aliquots suitable for testing one assay plate each, 30mL of reagent per aliquot can be dispensed and frozen down.
  
  Protein Dilution Buffer
  Once thawed, the Protein Dilution Buffer can be stored at 4°C for up to 4 weeks. For longer storage (up to the expiration date listed in the kit certificate of analysis), the reagent should be aliquoted and stored at -20°C until needed. Avoid multiple freeze-thaw cycles. To make aliquots suitable for testing one assay plate each, 10 mL of reagent per aliquot should be dispensed and frozen down. This amount may vary depending on your stock sample concentrations and should be adjusted accordingly.
  
  Recombinant Human VEGF165 Control Agonist
  Store at -20°C until ready to use. Centrifuge the vial prior to opening to maximize recovery. Reconstitute to a concentration of 100 μg/mL by adding 100 μL of Protein Dilution Buffer. Reconstituted ligand is stable for 12 months at -20 to -80°C, or 1 week at 2-8 °C.
  
  96-well Tissue Culture Treated Plates
  Store at room temperature.
• Size: 2 x 96-well