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AGTRL1 Activated GPCR Internalization Assay Kit

Cat.No. : Kit-2190
Product Overview : Activated GPCR Internalization cells are engineered to co-an untagged GPCR, Enzyme Acceptor (EA) tagged β-Arrestin, and a ProLink (PK) tag localized to the endosomes. Activation of the GPCR induces β-Arrestin recruitment, followed by internalization of the Receptor/Arrestin-EA complex in PK-tagged endosomes. This forces complementation of the two β-galactosidase enzyme fragments (EA and PK), forming functional enzyme that hydrolyzes substrate to generate a chemiluminescent signal. These cells have been modified to prevent long term propagation and expansion using a proprietary compound that has no apparent effect on assay performance.
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Storage : Short term (2 weeks): Store in vapor phase of liquid N2
Size : 100 dp
(1 x 96-well)/200 dp
(2 x 96-well)/1000 dp
(10 x 96-well)

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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What are the structural features of AGTRL1 that contribute to its unique ligand binding and activation mechanism? 03/09/2022

AGTRL1, like other GPCRs, possesses a seven-transmembrane helical bundle domain, which accommodates its ligand-binding pocket and interacts with angiotensin II. Additionally, specific amino acid residues in the receptor's binding site determine its selectivity for angiotensin II and related ligands.

How is AGTRL1 involved in the regulation of sodium and water balance? 01/21/2022

AGTRL1 activation influences the secretion of aldosterone, a hormone that regulates sodium and water balance in the kidneys.

Are there any AGTRL1 knockout animal models used in research? 09/02/2021

Yes, AGTRL1 knockout animal models, such as mice lacking the AGTRL1 gene, have been valuable tools for studying the protein's functions in various physiological processes.

What is the tissue distribution of AGTRL1 in the human body? 05/12/2021

AGTRL1 is widely expressed in various tissues, including the cardiovascular system, brain, kidney, and adrenal glands.

What are the downstream signaling pathways activated by AGTRL1? 02/18/2021

AGTRL1 activates various intracellular signaling pathways, including the phospholipase C (PLC)-inositol trisphosphate (IP3) pathway and mitogen-activated protein kinase (MAPK) pathway.

Does AGTRL1 have any non-blood-pressure-related functions? 07/07/2020

Yes, AGTRL1 has been implicated in various other physiological processes, such as cell proliferation, cell migration, and angiogenesis.

Are there any natural compounds known to modulate AGTRL1 activity? 12/03/2019

Some studies have suggested that certain natural compounds, such as flavonoids and polyphenols, might affect AGTRL1 activity, but further investigation is necessary.

How does AGTRL1 contribute to hypertension? 11/29/2019

AGTRL1's activation by angiotensin II leads to vasoconstriction and increased blood pressure, contributing to hypertension.

Can AGTRL1 play a role in cancer progression? 09/14/2018

There is emerging evidence suggesting that AGTRL1 may be involved in cancer development and progression, particularly in angiogenesis and tumor growth.

How does AGTRL1 interact with other components of the renin-angiotensin system to regulate blood pressure and fluid balance? 06/26/2018

AGTRL1 interacts with angiotensin-converting enzyme (ACE) and angiotensin II type 1 receptor (AT1R) to modulate blood pressure. ACE converts angiotensin I to angiotensin II, which subsequently binds to AGTRL1 and AT1R, leading to vasoconstriction and increased aldosterone secretion.

Customer Reviews (5)

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Reviews
08/12/2021

    Wow, this protein is incredible! It has really improved my experiments.

    01/26/2021

      My colleagues and I are thrilled with the results we achieved using this protein.

      06/21/2020

        The protein worked like magic in my lab. Highly recommended!

        10/03/2019

          The ordering process was smooth, and the recombinant proteins arrived on time and intact

          11/09/2018

            Quick delivery and secure packaging ensured my recombinant proteins arrived in perfect condition.

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