alpha-Ketoglutarate Assay Kit is used for quantifying alpha-KG.
α-Ketoglutarate (α-KG) is a key intermediate in the Krebs cycle, coming after isocitrate and before succinyl CoA. Anaplerotic reactions replenish the cycle by synthesizing α-KG from transamination of glutamate, or through the action of glutamate dehydrogenase. α-KG is an important nitrogen transporter. Being a key intermediate, it is one of the organic acids measured in newborns as an indicator of inborn errors of metabolism. The α-Ketoglutarate Assay Kit provides a simple, sensitive and rapid means for quantifying α-KG in a variety of samples. In the assay, α-ketoglutarate is transaminated with the generation of pyruvate which is utilized to convert a nearly colorless probe to both color (λmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The α-Ketoglutarate Assay Kit is useful for detecting α-ketoglutarate in the range of 0.01 to 10 nmoles.
For research use only (RUO)
Store kit at -20°C, protect from light. Warm α-KG Assay Buffer to room temperature before use. Briefly centrifuge all small vials prior to opening.
α-KG Assay Buffer 25 mlα-KG OxiRed Probe (in DMSO) 0.2 mlα-KG Converting Enzyme (lyophilized) 1 vialα-KG Development Enzyme Mix (lyophilized) 1 vialα-KG Standard (10 µmol, lyophilized) 1 vial
Compatible Sample Types:
Biological Fluid, Cell Culture Supernatant, Plasma, Serum, Tissue Culture Supernatant, Urine