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Home > Products > Assay Kits > Metabolism Kits > alpha-Ketoglutarate Assay Kit

alpha-Ketoglutarate Assay Kit

"α-KG" Related Products.
Product Overview:
alpha-Ketoglutarate Assay Kit is used for quantifying alpha-KG.
α-Ketoglutarate (α-KG) is a key intermediate in the Krebs cycle, coming after isocitrate and before succinyl CoA. Anaplerotic reactions replenish the cycle by synthesizing α-KG from transamination of glutamate, or through the action of glutamate dehydrogenase. α-KG is an important nitrogen transporter. Being a key intermediate, it is one of the organic acids measured in newborns as an indicator of inborn errors of metabolism. The α-Ketoglutarate Assay Kit provides a simple, sensitive and rapid means for quantifying α-KG in a variety of samples. In the assay, α-ketoglutarate is transaminated with the generation of pyruvate which is utilized to convert a nearly colorless probe to both color (λmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The α-Ketoglutarate Assay Kit is useful for detecting α-ketoglutarate in the range of 0.01 to 10 nmoles.
Target Species:
For research use only (RUO)
Store kit at -20°C, protect from light. Warm α-KG Assay Buffer to room temperature before use. Briefly centrifuge all small vials prior to opening.
Kit Components:
α-KG Assay Buffer 25 mlα-KG OxiRed Probe (in DMSO) 0.2 mlα-KG Converting Enzyme (lyophilized) 1 vialα-KG Development Enzyme Mix (lyophilized) 1 vialα-KG Standard (10 µmol, lyophilized) 1 vial
Detection method:
Colorimetric, Fluorometric
Compatible Sample Types:
Biological Fluid, Cell Culture Supernatant, Plasma, Serum, Tissue Culture Supernatant, Urine

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

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