|Product Overview:||Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. Apo-BrdU-IHC; Kit is a two-color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labeling) assay for labeling DNA breaks to detect apoptotic cells by immunohistochemistry. The kit contains positive/negative control slides for assessing reagent performance; reaction and blocking buffers for processing individual steps in the assay; proteinase K; terminal deoxynucleotidyl tranferase enzyme (TdT), bromodeoxyuridine triphosphate (Br-dUTP), biotin labeled antiBrdU antibody for labeling DNA breaks, horseradish peroxidase streptavidin conjugate, DAB, H2O2/Urea tablets for color generation and methyl green solution for counter staining the cells.|
|Applications:||The TUNEL-based immunohistochemical staining kit provides complete reagents including positive and negative control slides for convenient detection of DNA fragmentation.|
|Kit Components:||Control Slides-pos/neg; Blocking Buffer; H2O2/Urea Tablets; Protease K; DAB Tablets; TdT Enzymes; Br-dUTP; 200X Conjugate; 5X Reaction Buffer; Anti-BrdU-Biotin Antibody; Methyl Green|
|Features & Benefits:||Simple procedure; takes ~ 3 hours;
Fast and convenient;
The assay is sensitive and stable;
The TUNEL-based assay kit provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells and tissue sections.
Price does not include shipping and packaging costs.Request quote for final price.