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Home > Products > Assay Kits > Activity Kits > β-Secretase Activity Fluorometric Assay Kit

β-Secretase Activity Fluorometric Assay Kit

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Product Overview:
A sensitive fluorogenic assay kit for the determination of β-secretase (BACE) activity (Excitation max: 335-355 nm; Emission max: 495-501 nm). β-Secretase is a transmembrane aspartyl protease that cleaves membrane-bound amyloid precursor protein.
1 kit/100 Tests
The Calbiochem β-Secretase Activity Assay Kit, Fluorogenic, is intended for measuring the β-secretase (BACE) activity in cell lysates, tissue extracts, or purified enzyme preparations.
Storage: -20°C Storage Conditions: Upon arrival store the entire contents of the kit at -20°C. Following reconstitution of the Active β-Secretase, aliquot and freeze (-70°C) to avoid loss of activity. Following initial thaw of the kit contents, store the Extraction Buffer and 2X Reaction Buffer at 4°C.Protect from Light: Protect from light Avoid freeze/thaw: Avoid freeze/thaw Do not freeze: No
Kit Components:
Extraction Buffer: 1 bottle, 25 ml2X Reaction Buffer: 1 vial, 10 mlβ-Secretase Substrate: 1 vial, 200 µl, supplied in DMSOActive β-Secretase: 1 vial, lyophilizedβ-Secretase Inhibitor: 1 vial, 10 µl, supplied in DMSO
Detection method:
Compatible Sample Types:
Cell lysates, tissue extracts, purified BACE

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

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