Cholinesterase Activity Assay Kit (Colorimetric)

• Cat.No.: Kit-1070

Specification

• Applications: Measurement of ChE activity in various tissues/cells
  Screening of ChE inhibitors
• Storage: Store Kit at -20°C, protect from light. Briefly centrifuge small vials prior to opening. Read entire protocol before performing the assay.
  ChE Assay Buffer: Store at 4 ºC or -20 ºC. Bring to room temperature before use.
  AChE Substrate: Reconstitute in 100 µl ChE Assay Buffer. Store at -20ºC. Use within two months.
  BChE Substrate: Store at -20°C, protect from light. Bring to room temperature before use.
  Acetylcholinesterase: Reconstitute with 100 µl of ChE Assay Buffer. Aliquot and store at -20 ºC. Use within two months.
  Butyrylcholinesterase: Reconstitute with 20 µl ChE Assay Buffer. Store at -20 ºC. Use within two months.
  BChE Inhibitor: Reconstitute BChE Inhibitor in 150 µl dH2O. Vortex intensively at room temperature to facilitate solubilization. Aliquot and store at -20ºC. Bring to room temperature before use. Use it within two months.
  DTNB Solution: Dissolve 1 vial of DTNB with 625 µl ChE Assay Buffer. Each vial can be used to carry out up to 50 reactions. Dissolve
  vial contents when needed. Store at -20ºC. Use within two months.
  TNB Standard: Dissolve in 1 ml of ChE Assay Buffer to generate 2.5 mM TNB Standard. Use within two months.
• Size: 100 assays