Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-097-1828   Email:
Home > Products > Assay Kits > Activity Kits > cGMP Direct Immunoassay Kit

cGMP Direct Immunoassay Kit

"cGMP" Related Products.
Product Overview:
cGMP Direct Immunoassay Kit is used for determing cGMP level.
Adenosine and guanosine 3",5"-cyclic monophosphate (cAMP and cGMP) are important "second messengers" involved in many physiological processes. The cGMP Direct Immunoassay Kit provides a direct competitive immunoassay for sensitive and quantitative determination of cGMP level in biological samples. The kit utilizes the recombinant Protein G coated plate to anchor cGMP polyclonal antibody. cGMP-HRP conjugates directly competes with cGMP from samples for binding to the cGMP specific antibody on the plate. After incubation and washing, the amount of cGMP-HRP bound to plate can easily be determined by reading OD450 nm. The intensity of OD450 nm is inversely proportional to the concentration of cGMP in samples. The kit provides a new acetylation procedure that improves detection signal significantly. The kit can detect 0.04 -10 pmol/well (0.008 - 2 µM) cGMP samples.
Target Species:
For research use only (RUO)
The kit should be stored at -20°C. After reconstitution, some components may be stored at 4°C as instructedabove, stable for up to 1- 2 months.Component Storage10X cGMP Assay Buffer 4°CStandard cGMP (10 nmol) -20°CNeutralizing Buffer 4°CAcetylating Reagent A 4°CAcetylating Reagent B* 4°CAnti-cGMP pAb/BSA -20°CcGMP-HRP/BSA -20°CHRP Developer 4°CProtein G Coated Plate -20°C*Note: Acetylating Reagent B is very volatile and hence the vial has to be tightly capped and stored only at 4°C.
Kit Components:
10X cGMP Assay Buffer 25 mLStandard cGMP (10 nmol) 1 vialNeutralizing Buffer 7.5 mLAcetylating Reagent A 0.75 mLAcetylating Reagent B 1.5 mLAnti-cGMP pAb/BSA 1 vialcGMP-HRP/BSA 1 vialHRP Developer 10 mLProtein G Coated Plate 1 each
Detection method:
Compatible Sample Types:
Biological Fluid, Cell Lysate, Culture Medium, Plasma, Serum, Tissue Lysate, Urine

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127

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