Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Activity Kits > cGMP Direct Immunoassay Kit

cGMP Direct Immunoassay Kit

   Share
"cGMP" Related Products.
Cat.No.:
Kit-0212
Product Overview:
cGMP Direct Immunoassay Kit is used for determing cGMP level.
Description:
Adenosine and guanosine 3",5"-cyclic monophosphate (cAMP and cGMP) are important "second messengers" involved in many physiological processes. The cGMP Direct Immunoassay Kit provides a direct competitive immunoassay for sensitive and quantitative determination of cGMP level in biological samples. The kit utilizes the recombinant Protein G coated plate to anchor cGMP polyclonal antibody. cGMP-HRP conjugates directly competes with cGMP from samples for binding to the cGMP specific antibody on the plate. After incubation and washing, the amount of cGMP-HRP bound to plate can easily be determined by reading OD450 nm. The intensity of OD450 nm is inversely proportional to the concentration of cGMP in samples. The kit provides a new acetylation procedure that improves detection signal significantly. The kit can detect 0.04 -10 pmol/well (0.008 - 2 µM) cGMP samples.
Target Species:
Mammals
Usage:
For research use only (RUO)
Storage:
The kit should be stored at -20°C. After reconstitution, some components may be stored at 4°C as instructedabove, stable for up to 1- 2 months.Component Storage10X cGMP Assay Buffer 4°CStandard cGMP (10 nmol) -20°CNeutralizing Buffer 4°CAcetylating Reagent A 4°CAcetylating Reagent B* 4°CAnti-cGMP pAb/BSA -20°CcGMP-HRP/BSA -20°CHRP Developer 4°CProtein G Coated Plate -20°C*Note: Acetylating Reagent B is very volatile and hence the vial has to be tightly capped and stored only at 4°C.
Kit Components:
10X cGMP Assay Buffer 25 mLStandard cGMP (10 nmol) 1 vialNeutralizing Buffer 7.5 mLAcetylating Reagent A 0.75 mLAcetylating Reagent B 1.5 mLAnti-cGMP pAb/BSA 1 vialcGMP-HRP/BSA 1 vialHRP Developer 10 mLProtein G Coated Plate 1 each
Detection method:
Colorimetric
Compatible Sample Types:
Biological Fluid, Cell Lysate, Culture Medium, Plasma, Serum, Tissue Lysate, Urine


Online Inquiry    
Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
Please input "biomart" as verification code.
Please review Creative Biomart's privacy policy for more information.
Price does not include shipping and packaging costs.Request quote for final price.

If you have any other questions, please call
+1-631-559-9269.

online inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

facebook twitter linkedin google plus google plus

Join 10 other subscribers

24x7
CUSTOMER SERVICE
Easy access to products and services you need from our library via powerful searching tools
Copyright © 2007 - 2016 Creative Biomart. All Rights Reserved.