cAMP Direct Immunoassay Detection Kit (Fluorometric)

• Cat.No.: Kit-0866
• Product Overview: cAMP Direct Immunoassay Detection Kit (Fluorometric) provides a sensitive method for detecting adenylate cyclase activity in biochemical or cell-based assay systems. Compared to other ELISA cAMP assay kits, this kit eliminates the tedious acetylation step. The kit uses AbRed Indicator as a fluorimetric substrate to quantify the HRP activity. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation. The fluorescent product formed is proportional to the activity of HRP-cAMP conjugate. This kit is based on the competition between HRP-labeled cAMP and free cAMP for a fixed number of cAMP antibody binding sites. HRP-cAMP is displaced from the HRP-cAMP/anti-cAMP antibody complex by unlabeled free cAMP. In the absence of cAMP, HRP-cAMP conjugate is bound to anti-cAMP antibody exclusively. However, the unlabeled free cAMP in the test samples competes for anti-cAMP antibody with the HRP-cAMP antibody conjugate, therefore inhibits the binding of HRP-cAMP to anti-cAMP antibody.

Specification

• Size: 1 x 96 tests
• Applications: Functional Studies more details
• Storage: Store at +4°C.