"Caspase-4" Related Products

Caspase-4 Colorimetric Assay Kit

Cat.No.: Kit-1118
Description: Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-4 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEVD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate LEVD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm.
Applications: Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
Storage: -20°C
Shipping: Gel Pack
Kit Components: Cell Lysis Buffer; 2X Reaction Buffer; LEVD-pNA (4 mM); DTT (1 M); Dilution Buffer
Target Species: Mammalian
Detection method: Absorbance (400or 405 nm)
Features & Benefits: Simple one-step procedure; takes only 1-2 hours;
Fast and convenient;
Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in LEVD-dependent Caspase activity.

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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