"CBS" Related Products

Expand
Expand

Cystathionine β Synthase Activity Assay Kit (Fluorometric)

Cat.No.: Kit-1113
Description: Cystathionine β Synthase (EC 4.2.1.22, CβS) is a PLP-dependent enzyme that catalyzes the formation of H2S and cystathionine when using cysteine and homocysteine as substrates. Known for its role in human sulfur metabolism, mutations in the gene encoding CβS can result in high concentrations of homocysteine in plasma. Elevated circulating concentrations of homocysteine result in genetic disorders including Homocysteinuria and Down syndrome. Specifically, Homocysteinuria is an autosomal recessive disease with clinical manifestations including mental retardation, thromboembolism and connective tissue defects. Hydrogen sulfide, CβS product, is an important gaseous mediator, like nitric oxide, that has significant effects on the immunological, neurological, cardiovascular and pulmonary systems ofmammals. Cystathione β Synthase Assay Kit utilizes cysteine and homocysteine as substrates to produce H2S. Hydrogen sulfide reacts with the azido-functional group of the fluorescent probe yielding a fluorescent amino group (Ex/Em = 368/460 nm). The assay is highly sensitive, has a simple easy-to-follow protocol, and can detect as low as 1.45 mU of CβS activity.
Applications: Rapid assessment of native/recombinant CβSand CβS activity in mammalian samples
Storage: -20°C
Shipping: Gel Pack
Size: 100 assays
Kit Components: CβS Assay Buffer; CβS Probe (in DMSO); CβS Substrate; Cofactor 1; Cofactor 2; Reducing Agent; AMC Standard (in DMSO, 1 mM); CβS Positive Control
Target Species: Eukaryotes
Detection method: Fluorescence(Ex/Em 368/460 nm)
Features & Benefits: Simple, convenient, highly sensitive;
Fluorescent assay enables easy determination of CβS activity in a variety of biological samples;
Kit includes a stable CβS positive control

Online Inquiry

Note: There will be extra charge for optional service!
Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information


Price does not include shipping and packaging costs.Request quote for final price.

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.