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Recombinant Human ACAT2, His-tagged

Cat.No. : ACAT2-776H
Product Overview : Recombinant human ACAT2 protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW =45.4 kDa (433aa) confirmed by MALDI-TOF.
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  • Gene Information
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Cat. No. : ACAT2-776H
Description : ACAT2 is an enzyme involved in lipid metabolism. It participates in lipoprotein assembly, catalyzing cholesterol esterification in mammalian cells. ACAT2 is an integral membrane protein that localizes to the endoplasmic reticulum of human intestinal cells. ACAT2 deficiency contributes to severe mental retardation and hypotonus.
Source : E.coli.
Sequences Of Amino Acids : MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMNAG SDPVVIVSAA RTIIGSFNGA LAAVPVQDLG STVIKEVLKR ATVAPEDVSE VIFGHVLAAG CGQNPVRQAS VGAGIPYSVP AWSCQMICGS GLKAVCLAVQ SIGIGDSSIV VAGGMENMSK APHLAYLRTG VKIGEMPLTDSILCDGLTDA FHNCHMGITA ENVAKKWQVS REDQDKVAVL SQNRTENAQK AGHFDKEIVP VLVSTRKGLI EVKTDEFPRH GSNIEAMSKL KPYFLTDGTG TVTPANASGI NDGAAAVVLM KKSEADKRGL TPLARIVSWS QVGVEPSIMG IGPIPAIKQA VTKAGWSLED VDIFEINEAF AAVSAAIVKE LGLNPEKVNI EGGAIALGHP LGASGCRILV TLLHTLERMG RSRGVAALCI GGGMGIAMCV QRE
Purity : > 95% by SDS – PAGE.
Form : Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.1M NaCl, 1mM DTT, 10% glycerol.
Concentration : 1 mg/ml (determined by Bradford assay).
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Gene Name : ACAT2 acetyl-CoA acetyltransferase 2 [ Homo sapiens ]
Synonyms : ACAT2; acetyl-CoA acetyltransferase 2; acetyl-Coenzyme A acetyltransferase 2; Acetyl-CoA acetyltransferase, cytosolic; Cytosolic acetoacetyl-CoA thiolase; Acetyl-CoA transferase-like protein; EC 2.3.1.9; OTTHUMP00000017527; Cytosolic acetoacetyl-CoA thiolase; acetoacetyl Coenzyme A thiolase; acetyl-Coenzyme A acetyltransferase 2; acetyl-Coenzyme A acetyltransferase 2 (acetoacetyl Coenzyme A thiolase)
Gene ID : 39
mRNA Refseq : NM_005891
Protein Refseq : NP_005882
MIM : 100678
UniProt ID : Q9BWD1
Chromosome Location : 6q25.3
Pathway : Benzoate degradation via CoA ligation; Butanoate metabolism; Fatty acid metabolism; Lysine degradation; Metabolic pathways; Propanoate metabolism; Pyruvate metabolism; Synthesis and degradation of ketone bodies; Terpenoid backbone biosynthesis; Tryptophan metabolism; Valine, leucine and isoleucine degradation
Function : acetyl-CoA C-acetyltransferase activity; acyltransferase activity; protein binding; transferase activity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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How to measure the purity of recombinant ACAT2 protein? 02/05/2023

Typically, protein purity is measured using techniques such as SDS-PAGE, Western blotting, or HPLC.

Is there any discount for recombinant human ACAT2 protein (ACAT2-144H)? 12/23/2022

We are offering a 10% discount on all products for a limited time. The clock is ticking down. Be sure to take advantage of our holiday promotions!

How is the quality of recombinant ACAT2 protein ensured? 12/12/2022

Creative BioMart uses a variety of quality control measures to ensure the purity, identity, and activity of our products. These may include analytical testing such as SDS-PAGE, Western blotting, and ELISA, as well as functional assays to confirm activity. We can also perform endotoxin testing or use other methods to ensure the product is free from contaminants.

Are there any precautions or safety concerns to be aware of when working with recombinant ACAT2 protein? 03/14/2022

Like all proteins, recombinant ACAT2 protein should be handled with appropriate safety precautions, such as wearing gloves and avoiding direct contact with skin or mucous membranes. It is also important to follow good laboratory practices and to dispose of any waste materials properly.

What is the recommended concentration and volume of recombinant ACAT2 protein for use in assays or experiments? 02/21/2022

The recommended concentration and volume of recombinant ACAT2 protein can vary depending on the specific assay or experiment being performed. Typically, the protein is dissolved in a buffer solution at a desired concentration (e.g. 0.1-1 mg/mL) and then used as needed. It is important to ensure that the protein concentration is appropriate for the intended use, as high concentrations may result in nonspecific binding or other issues.

What are the potential applications of recombinant ACAT2 protein? 12/12/2021

Recombinant ACAT2 protein can be used in a variety of research applications, including studying the function of ACAT2 in lipid metabolism and cholesterol homeostasis, as well as in drug discovery efforts related to cardiovascular disease, obesity, and other metabolic disorders. It may also be used as a reference or standard in diagnostic tests for certain diseases.

How are the expression and activity of ACAT2 regulated in the body? 08/22/2021

The expression and activity of ACAT2 are regulated by a variety of factors, including dietary cholesterol intake, hormonal signals, and transcriptional and post-transcriptional mechanisms. Studies have identified several transcription factors that bind to the ACAT2 promoter region and regulate its expression, as well as post-transcriptional mechanisms such as alternative splicing and miRNA-mediated regulation.

Are there any alternative sources of ACAT2 protein, such as natural sources? 03/17/2021

In addition to recombinant ACAT2 protein, there may be natural sources of ACAT2 protein, such as from animal or human tissues. However, these sources may be more difficult to obtain and may have variable purity or activity. It is important to evaluate the quality and activity of these alternative sources before use in experiments or assays.

What are the potential therapeutic applications of targeting ACAT2? 10/12/2020

Inhibition of ACAT2 has been proposed as a potential therapeutic strategy for the treatment of cardiovascular disease, as well as other metabolic disorders such as nonalcoholic fatty liver disease (NAFLD) and type 2 diabetes. Several small molecule inhibitors of ACAT2 have been developed and are currently undergoing preclinical and clinical evaluation.

What is the role of ACAT2 in lipid metabolism and its association with atherosclerosis? 09/18/2020

ACAT2 is a key enzyme involved in the conversion of free cholesterol to cholesterol esters, which are stored in lipid droplets in cells. Dysregulation of ACAT2 activity has been implicated in the development of atherosclerosis and other metabolic disorders. Studies have shown that inhibition of ACAT2 can reduce atherosclerotic plaque formation in animal models, suggesting that ACAT2 may be a potential therapeutic target for the prevention and treatment of cardiovascular disease.

Customer Reviews (3)

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Reviews
01/02/2023

    The purity and quality of Creative BioMart's recombinant human ACAT2 protein is unmatched. I have tried other protein products in the past, but this one is by far the best.

    12/12/2022

      The recombinant human ACAT2 protein is of high quality and purity, and it has worked perfectly in my experiments.

      12/12/2021

        The recombinant human ACAT2 protein from Creative BioMart is highly pure and stable, and I have had great success using it in my assays.

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