Recombinant Human APEX1, T7-tagged
Cat.No. : | APEX1-955H |
Product Overview : | Recombinant human APEX1 protein, fused to T7-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
- Specification
- Gene Information
- Related Products
Cat. No. : | APEX1-955H |
Description : | APEX1, also known as REF1 or APE1 (Apurinic / apyrimidinic endonuclease), is responsible for the incision of DNA basic sites during base excision repair. It has also been shown to stimulate the DNA binding activity of numerous transcription factors that are involved in cancer promotion and progression. APEX1 is part of the cellular response to oxidative stress and protects cells from the genotoxic and cytotoxic effect of oxidizing agents. |
Source : | E.coli. |
Form : | Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 2mM DTT, 10% glycerol. |
Molecular Weight : | 36.9 kDa (332aa) confirmed by MALDI-TOF. |
Purity : | > 90% by SDS – PAGE. |
Concentration : | 1 mg/ml (determined by Bradford assay). |
Sequences Of Amino Acids : | MASMTGGQQM GRGSMPKRGK KGAVAEDGDE LRTEPEAKKS KTAAKKNDKE AA GEGPALYE DPPDQKTSPS GKPATLKICS WNVDGLRAWI KKKGLDWVKE EAP DILCLQE TKCSENKLPA ELQELPGLSH QYWSAPSDKE GYSGVGLLSR QCP LKV SYGI GEEEHDQEGR VIVAEFDSFV LVTAYVPNAG RGLVRLEYRQ RWDEAFRKFL KGL ASRKPLV LCGDLNVAHE EIDLRNPKGN KKNAGFTPQE RQGFGELLQA VPL ADSFRHL YPNTPYAYTF WTYMMNARSK NVGWRLDYFL LSHSLLPALC DSK IRSK ALG SDHCPITLYL AL. |
Storage : | Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles. |
Pathways : | Base excision repair; DNA Repair |
Gene Name : | APEX1 APEX nuclease (multifunctional DNA repair enzyme) 1 [ Homo sapiens ] |
Synonyms : | APEX nuclease (multifunctional DNA repair enzyme) 1; APE; APX; APE1; APEN; APEX; HAP1; REF1; APEX1; DNA-(apurinic or apyrimidinic site) lyase; AP lyase; protein REF-1; redox factor 1; AP endonuclease 1; AP endonuclease class I; apurinic/apyrimidinic exonuclease; multifunctional DNA repair enzyme; apurinic-apyrimidinic endonuclease 1; apurinic/apyrimidinic (abasic) endonuclease; deoxyribonuclease (apurinic or apyrimidinic); EC 4.2.99.18; APEX nuclease (multifunctional DNA repair enzyme);APE-1; REF-1; APEX nuclease 1; APEX nuclease |
Gene ID : | 328 |
mRNA Refseq : | NM_001641 |
Protein Refseq : | NP_001632 |
MIM : | 107748 |
UniProt ID : | P27695 |
Chromosome Location : | 14q11.2-q12 |
Function : | 3"-5" exonuclease activity; DNA binding; DNA-(apurinic or apyrimidinic site) lyase activity; endodeoxyribonuclease activity; endonuclease activity; lyase activity; metal ion binding; oxidoreductase activity; phosphodiesterase I activity; phosphoric diester hydrolase activity; protein binding; ribonuclease H activity; transcription coactivator activity; transcription corepressor activity; uracil DNA N-glycosylase activity |
Products Types
◆ Recombinant Protein | ||
APEX1-1330H | Recombinant Human APEX1 Protein (32-318 aa), His-tagged | +Inquiry |
APEX1-2705H | Recombinant Human APEX1 protein(11-90 aa), C-His-tagged | +Inquiry |
APEX1-354H | Recombinant Human APEX1 Protein, His (Fc)-Avi-tagged | +Inquiry |
Apex1-623M | Recombinant Mouse Apex1 Protein, MYC/DDK-tagged | +Inquiry |
APEX1-2497H | Recombinant Human APEX1 protein(Pro2-Leu318), His-tagged | +Inquiry |
◆ Lysates | ||
APEX1-8797HCL | Recombinant Human APEX1 293 Cell Lysate | +Inquiry |
APEX1-8796HCL | Recombinant Human APEX1 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
- Q&As
- Reviews
Q&As (20)
Ask a questionThe recombinant APEX1 protein may have broader application prospects in future clinical applications, especially in the treatment of diseases related to DNA damage.
The recombinant APEX1 protein can be used in scientific research to study the DNA repair pathway and the pathogenesis of related diseases.
The APEX1 is a DNA repair enzyme that can repair DNA bases damaged by oxidation.
The recombinant APEX1 protein can perform biological functions in the body and participate in the repair process of DNA.
In general, there are no serious side effects from the use of APEX1 , but specific side effects need to be evaluated based on actual use.
Yes, the recombinant APEX1 protein has antioxidant effects and can help reduce the damage to cells caused by oxidative stress.
APEX1 is not currently widely used in gene therapy, but it may have potential applications in related studies.
Yes, some studies have shown that the APEX1 can improve the occurrence and development of cardiovascular disease.
The study and detection of APEX1 can be performed using conventional immunological and biochemical methods.
The synthesis methods of APEX1 mainly include prokaryotic expression system and eukaryotic expression system.
The antioxidant and DNA repair effects of APEX1 make it a potential candidate molecule for anticancer drugs.
Yes, some studies have shown that the presence of recombinant APEX1 protein can improve the efficiency of DNA repair.
The therapeutic effect of APEX1 can be evaluated through cell experiments, animal experiments and clinical trials.
Purified samples of APEX1 can be obtained by protein expression and purification techniques.
APEX1 can be used clinically to treat diseases related to DNA damage, such as cancer.
The APEX1 is relevant for the treatment of diseases related to DNA damage such as cancer and neurological diseases.
No studies have shown that recombinant APEX1 can extend life span.
When using APEX1 , it is necessary to pay attention to the appropriate dosage and method, and follow the relevant experimental procedures.
There are currently no commercially available APEX1 drugs, but research is ongoing.
Yes, the recombinant APEX1 protein is associated with cancer because its abnormal function in DNA damage repair can lead to the development of cancer.
Customer Reviews (5)
Write a reviewHigh clearance of APELA allow the drug to act more quickly and reach the therapeutic goal quickly.
Zero pollution in the production process has a positive impact on the environment.
Easy to use, good results, great results.
The sensitivity of APELA in Western blot is very high, and clear bands can be obtained even at low concentrations, indicating that it has the characteristics of high sensitivity and low background noise.
It still maintains stable performance after a long time of use, showing excellent product quality.
Ask a Question for All APEX1 Products
Required fields are marked with *
My Review for All APEX1 Products
Required fields are marked with *
Inquiry Basket