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Recombinant Human APEX1, T7-tagged

Cat.No. : APEX1-955H
Product Overview : Recombinant human APEX1 protein, fused to T7-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
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Cat. No. : APEX1-955H
Description : APEX1, also known as REF1 or APE1 (Apurinic / apyrimidinic endonuclease), is responsible for the incision of DNA basic sites during base excision repair. It has also been shown to stimulate the DNA binding activity of numerous transcription factors that are involved in cancer promotion and progression. APEX1 is part of the cellular response to oxidative stress and protects cells from the genotoxic and cytotoxic effect of oxidizing agents.
Source : E.coli.
Form : Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 2mM DTT, 10% glycerol.
Molecular Weight : 36.9 kDa (332aa) confirmed by MALDI-TOF.
Purity : > 90% by SDS – PAGE.
Concentration : 1 mg/ml (determined by Bradford assay).
Sequences Of Amino Acids : MASMTGGQQM GRGSMPKRGK KGAVAEDGDE LRTEPEAKKS KTAAKKNDKE AA GEGPALYE DPPDQKTSPS GKPATLKICS WNVDGLRAWI KKKGLDWVKE EAP DILCLQE TKCSENKLPA ELQELPGLSH QYWSAPSDKE GYSGVGLLSR QCP LKV SYGI GEEEHDQEGR VIVAEFDSFV LVTAYVPNAG RGLVRLEYRQ RWDEAFRKFL KGL ASRKPLV LCGDLNVAHE EIDLRNPKGN KKNAGFTPQE RQGFGELLQA VPL ADSFRHL YPNTPYAYTF WTYMMNARSK NVGWRLDYFL LSHSLLPALC DSK IRSK ALG SDHCPITLYL AL.
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Pathways : Base excision repair; DNA Repair
Gene Name : APEX1 APEX nuclease (multifunctional DNA repair enzyme) 1 [ Homo sapiens ]
Synonyms : APEX nuclease (multifunctional DNA repair enzyme) 1; APE; APX; APE1; APEN; APEX; HAP1; REF1; APEX1; DNA-(apurinic or apyrimidinic site) lyase; AP lyase; protein REF-1; redox factor 1; AP endonuclease 1; AP endonuclease class I; apurinic/apyrimidinic exonuclease; multifunctional DNA repair enzyme; apurinic-apyrimidinic endonuclease 1; apurinic/apyrimidinic (abasic) endonuclease; deoxyribonuclease (apurinic or apyrimidinic); EC 4.2.99.18; APEX nuclease (multifunctional DNA repair enzyme);APE-1; REF-1; APEX nuclease 1; APEX nuclease
Gene ID : 328
mRNA Refseq : NM_001641
Protein Refseq : NP_001632
MIM : 107748
UniProt ID : P27695
Chromosome Location : 14q11.2-q12
Function : 3"-5" exonuclease activity; DNA binding; DNA-(apurinic or apyrimidinic site) lyase activity; endodeoxyribonuclease activity; endonuclease activity; lyase activity; metal ion binding; oxidoreductase activity; phosphodiesterase I activity; phosphoric diester hydrolase activity; protein binding; ribonuclease H activity; transcription coactivator activity; transcription corepressor activity; uracil DNA N-glycosylase activity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (20)

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What is the development prospect of APEX1 in future clinical applications? 08/25/2022

The recombinant APEX1 protein may have broader application prospects in future clinical applications, especially in the treatment of diseases related to DNA damage.

What is the research value of APEX1 in scientific research? 08/16/2022

The recombinant APEX1 protein can be used in scientific research to study the DNA repair pathway and the pathogenesis of related diseases.

What is the relationship between APEX1 and DNA repair? 08/10/2022

The APEX1 is a DNA repair enzyme that can repair DNA bases damaged by oxidation.

Can APEX1 perform biological functions in vivo? 05/23/2022

The recombinant APEX1 protein can perform biological functions in the body and participate in the repair process of DNA.

What are the side effects of using APEX1 ? 04/09/2022

In general, there are no serious side effects from the use of APEX1 , but specific side effects need to be evaluated based on actual use.

Is recombinant APEX1 associated with antioxidant activity? 03/29/2022

Yes, the recombinant APEX1 protein has antioxidant effects and can help reduce the damage to cells caused by oxidative stress.

Can recombinant APEX1 be used in gene therapy? 12/04/2021

APEX1 is not currently widely used in gene therapy, but it may have potential applications in related studies.

Is there any research finding that APEX1 can improve cardiovascular disease? 08/11/2021

Yes, some studies have shown that the APEX1 can improve the occurrence and development of cardiovascular disease.

How can APEX1 be studied and detected? 07/16/2021

The study and detection of APEX1 can be performed using conventional immunological and biochemical methods.

What are the synthesis methods of APEX1 ? 06/14/2021

The synthesis methods of APEX1 mainly include prokaryotic expression system and eukaryotic expression system.

Is it possible for APEX1 to be a candidate molecule for anticancer drugs? 05/08/2021

The antioxidant and DNA repair effects of APEX1 make it a potential candidate molecule for anticancer drugs.

Is there any research showing that the APEX1 can improve the efficiency of DNA repair? 05/08/2021

Yes, some studies have shown that the presence of recombinant APEX1 protein can improve the efficiency of DNA repair.

How to evaluate the therapeutic effect of APEX1 ? 09/07/2020

The therapeutic effect of APEX1 can be evaluated through cell experiments, animal experiments and clinical trials.

How to obtain purified samples of APEX1 ? 07/03/2020

Purified samples of APEX1 can be obtained by protein expression and purification techniques.

What is the clinical application of APEX1 ? 05/01/2020

APEX1 can be used clinically to treat diseases related to DNA damage, such as cancer.

The APEX1 is relevant for the treatment of which diseases? 04/14/2020

The APEX1 is relevant for the treatment of diseases related to DNA damage such as cancer and neurological diseases.

Is there any research to prove that the APEX1 can extend life span? 03/26/2020

No studies have shown that recombinant APEX1 can extend life span.

What should I pay attention to when using APEX1 ? 09/10/2019

When using APEX1 , it is necessary to pay attention to the appropriate dosage and method, and follow the relevant experimental procedures.

Are there any APEX1 drugs already on the market? 09/06/2019

There are currently no commercially available APEX1 drugs, but research is ongoing.

Is recombinant APEX1 associated with cancer? 05/06/2019

Yes, the recombinant APEX1 protein is associated with cancer because its abnormal function in DNA damage repair can lead to the development of cancer.

Customer Reviews (5)

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Reviews
11/13/2022

    High clearance of APELA allow the drug to act more quickly and reach the therapeutic goal quickly.

    10/10/2021

      Zero pollution in the production process has a positive impact on the environment.

      06/14/2019

        Easy to use, good results, great results.

        04/27/2019

          The sensitivity of APELA in Western blot is very high, and clear bands can be obtained even at low concentrations, indicating that it has the characteristics of high sensitivity and low background noise.

          02/06/2019

            It still maintains stable performance after a long time of use, showing excellent product quality.

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