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Recombinant Human BCKDHA, His-tagged

Cat.No. : BCKDHA-962H
Product Overview : Recombinant human BCKDHA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
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  • Gene Information
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Cat. No. : BCKDHA-962H
Description : BCKDHA (branched-chain-keto acid dehydrogenase E1 component chain), also known as 2-oxoisovalerate dehydrogenase subunit alpha, belongs to the BCKDHA family. The branched-chain alpha-keto acid dehydrogenase complex is an innter mitochondrial enzyme complex that catalyzes the second major step in the catabolism of the branched-chain amino acids leucine, isoleucine, and valine. The BCKD complex consists of three catalytic components: a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1), a dihydrolipoyl transacylase (E2), and a dihydrolipoamide dehydrogenase (E3).
Source : E.coli.
Form : Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 30% glycerol, 0.2M NaCl.
Molecular Weight : 47.8kDa (421aa) confirmed by MALDI-TOF.
Purity : > 80% by SDS – PAGE.
Concentration : 0.25 mg/ml (determined by Bradford assay).
Sequences Of Amino Acids : MGSSHHHHHH SSGLVPRGSH MSSLDDKPQF PGASAEFIDK LEFIQPNVIS GIPI YRVMDR QGQIINPSED PHLPKEKVLK LYKSMTLLNT MDRILYESQR QGRI SFY MTN YGEEGTHVGS AAALDNTDLV FGQYREAGVL MYRDYPLELF MAQ CYGNISD LGK GRQMPVH YGCKERHFVT ISSPLATQIP QAVGAAYAAK RAN AN RVVIC YF G EGAASEG DAHAGFNFAA TLECPIIFFC RNNGYAISTP TSEQ YRGDGI AA RG PG YGIM SIRVDGNDVF AVYNATKEAR RRAVAENQPF LIEA MTYRIG HH STS DD S SA YRSVDEVNYW DKQDHPISRL RHYLLSQGWW DEE QEKAWRK QS RRK VME A F EQAERKPKPN PNLLFSDVYQ EMPAQLRKQQ ESLA RHLQTY GEHYPLDHFD K.
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Pathways : Metabolic pathways; Valine, leucine and isoleucine degradation; Metabolism of amino acids and derivatives
Gene Name : BCKDHA branched chain keto acid dehydrogenase E1, alpha polypeptide [ Homo sapiens ]
Synonyms : branched chain keto acid dehydrogenase E1, alpha polypeptide; MSU; MSUD1; OVD1A; BCKDE1A; FLJ45695; BCKDHA;2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial; BCKDH E1-alpha ; 2-oxoisovalerate dehydrogenase (lipoamide) ; branched-chain alpha-keto acid dehydrogenase E1 component alpha chain; Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain; branched chain keto acid dehydrogenase E1, alpha polypeptide (maple syrup urine disease); EC 1.2.4.4
Gene ID : 593
mRNA Refseq : NM_000709
Protein Refseq : NP_000700
MIM : 608348
UniProt ID : P12694
Chromosome Location : 19q13.1-q13.2
Function : 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) activity; alpha-ketoacid dehydrogenase activity; carboxy-lyase activity; metal ion binding; protein binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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