Recombinant Human BPHL protein, His-tagged

• Cat.No.: BPHL-3450H
• Product Overview: Recombinant Human BPHL(38-291aa) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Description: BPHL is a Serine hydrolase which Belongs to the AB hydrolase superfamily. This family catalyzes the hydrolytic activation of amino acid ester prodrugs of nucleoside analogs such as valacyclovir and valganciclovir. This protein is expressed at high levels in liver and kidney and at lower levels in heart, intestine and skeletal muscle. It plays a role in detoxification processes. BPHL is a specific alpha-amino acid ester hydrolase that prefers small, hydrophobic, and aromatic side chains and does not have a stringent requirement for the leaving group other than preferring a primary alcohol.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 1mM DTT, 50mM NaCl
• Molecular Mass: 31.1kDa (275aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MRDFPADFFER DAKDAVDLMK ALKFKKVSLL GWSDGGITAL IAAAKYPSYI HKMVIWGANA YVTDEDSMIY EGIRDVSKWS ERTRKPLEAL SVTSAKVAV NGVQLHYQQT GEGDHAVLLL PGMLGSGETD FGPQLKNLNK KLFTVVAWDP RGYGHSRPPDYGYDYFARTC EKWVDGIRQF KHLPDGNICR HLLPRVQCPA LIVHGEKDPL VPRFHADFIH KHVKGSRLHL MPEGKHNLHL RFADEFNKLAEDFLQ
• Purity: >95% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: BPHL biphenyl hydrolase-like (serine hydrolase) [ Homo sapiens ]
• Official Symbol: BPHL
• Synonyms: BPHL; biphenyl hydrolase-like (serine hydrolase); MCNAA; valacyclovir hydrolase; Bph rp; breast epithelial mucin associated antigen; valacyclovirase; biphenyl hydrolase-like protein; biphenyl hydrolase-related protein; breast epithelial mucin-associated antigen; BPH-RP; VACVASE; MGC41865; MGC125930
• Gene ID: 670
• mRNA Refseq: NM_004332
• Protein Refseq: NP_004323
• MIM: 603156
• UniProt ID: Q86WA6
• Chromosome Location: 6p25
• Function: hydrolase activity