Recombinant Human FKBP5 protein, His-tagged

• Cat.No.: FKBP5-2124H
• Product Overview: Recombinant Human FKBP5(Met1-Val457) fused with N-terminal his-tag (10 extra AA) was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: Filtered (0.4 μm) and lyophilized from solution 20 mM Tris buffer, 50 mM NaCl, pH 7.5.
• Molecular Mass: 52.5 kDa (calculated)
• AA Sequence: MKHHHHHHAS MTTDEGAKNN EESPTATVAE QGEDITSKKD RGVLKIVKRV GNGEETPMIG DKVYVHYKGK LSNGKKFDSS HDRNEPFVFS LGKGQVIKAW DIGVATMKKG EICHLLCKPE YAYGSAGSLP KIPSNATLFF EIELLDFKGE DLFEDGGIIR RTKRKGEGYS NPNEGATVEI HLEGRCGGRM FDCRDVAFTV GEGEDHDIPI GIDKALEKMQ REEQCILYLG PRYGFGEAGK PKFGIEPNAE LIYEVTLKSF EKAKESWEMD TKEKLEQAAI VKEKGTVYFK GGKYMQAVIQ YGKIVSWLEM EYGLSEKESK ASESFLLAAF LNLAMCYLKL REYTKAVECC DKALGLDSAN EKGLYRRGEA QLLMNEFESA KGDFEKVLEV NPQNKAARLQ ISMCQKKAKE HNERDRRIYA NMFKKFAEQD AKEEANKAMG KKTSEGVTNE KGTDSQAMEE EKPEGHV
• Endotoxin: < 1.0 EU/μg
• Purity: Purity as determined by densitometric image analysis: >90%
• Applications: ELISA, Western blotting
• Notes: BCA to determine quantity of the protein.
  SDS-PAGE to determine purity of the protein.
  LAL TEST to determine endotoxin level.
• Storage: Store the lyophilized protein at –80 centigrade. Lyophilized protein remains stable until the expiry date when stored at –80 centigrade. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80 centigrade for long term storage. Reconstituted protein can be stored at 4 centigrade for a week.
• Reconstitution: Add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.

Gene Information

• Gene Name: FKBP5 FK506 binding protein 5 [ Homo sapiens ]
• Official Symbol: FKBP5
• Synonyms: FKBP5; FK506 binding protein 5; peptidyl-prolyl cis-trans isomerase FKBP5; FKBP51; FKBP54; P54; PPIase; Ptg 10; FKBP-5; FKBP-51; rotamase; 51 kDa FKBP; FF1 antigen; PPIase FKBP5; HSP90-binding immunophilin; T-cell FK506-binding protein; androgen-regulated protein 6; 51 kDa FK506-binding protein 5; peptidylprolyl cis-trans isomerase; 54 kDa progesterone receptor-associated immunophilin; AIG6; Ptg-10; MGC111006
• Gene ID: 2289
• mRNA Refseq: NM_001145775
• Protein Refseq: NP_001139247
• MIM: 602623
• UniProt ID: Q13451
• Chromosome Location: 6p21.31
• Pathway: Glucocorticoid receptor regulatory network, organism-specific biosystem; TNF-alpha/NF-kB Signaling Pathway, organism-specific biosystem
• Function: FK506 binding; binding; heat shock protein binding; isomerase activity; peptidyl-prolyl cis-trans isomerase activity