Active Recombinant Human PKM2, His-tagged

• Cat.No.: PKM2-572H
• Product Overview: Recombinant Human full length Pyruvate kinase M2 (PKM2) also known as PKM1/M2, isoform a (GenBank Accession No. NM_002654), fused with N-terminal His-tag, was expressed in an E. coli expression system.

Specification

• Description: PKM1 and PKM2 are different splicing products of the PKM gene (exon 9 for PKM1 and exon 10 for PKM2). The M1 isoform (PKM1) is expressed in muscle and brain, and the M2 isoform is expressed during embryogenesis, in adipose tissue and pancreatic islets, and is the predominant form found in cancer cells.
• Source: E. coli
• Species: Human
• Tag: His
• Form: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole
• Bio-activity: ≥39 pmoles/min/µg.
• Molecular Mass: 58.9 kDa
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Storage: >6 months at –80°C

Gene Information

• Gene Name: PKM pyruvate kinase, muscle [ Homo sapiens (human) ]
• Official Symbol: PKM
• Synonyms: PKM; pyruvate kinase, muscle; PK3; PKM; TCB; OIP3; CTHBP; THBP1; MGC3932; Pyruvate kinase isozymes M1/M2; EC 2.7.1.40; Pyruvate kinase muscle isozyme; Pyruvate kinase 2/3; Cytosolic thyroid hormone-binding protein; Cytosolic thyroid hormone-bin ding protein; OPA-interacting protein 3; PK, muscle type; pyruvate kinase, muscle; thyroid hormone-binding protein, cytosolic
• Gene ID: 5315
• mRNA Refseq: NM_002654
• Protein Refseq: NP_002645
• MIM: 179050
• UniProt ID: P14618
• Chromosome Location: 15q22
• Pathway: Adenine ribonucleotide biosynthesis, IMP => ADP,ATP; Biosynthesis of amino acids; Glycogen storage diseases
• Function: ATP binding; MHC class II protein complex binding; magnesium ion binding