Recombinant Human Coagulation Factor VII (serum prothrombin conversion accelerator)

Cat. No.

F7-P042H

ProductOverview

RecombinantHuman F7 is expressed in hamster kidney cells and theresultant protein is 45.1 kDa (406aa).

Description

Recombinant humancoagulation Factor VIIa (rFVIIa), intended for promoting hemostasis byactivating the extrinsic pathway of the coagulation cascade.1 NovoSeven is avitamin K-dependent glycoprotein consisting of 406 amino acid residues.Cloned and expressed in hamster kidney cells, the protein is catalyticallyactive in a two-chain form.

Background

This gene encodescoagulation factor VII which is a vitamin K-dependent factor essential forhemostasis. This factor circulates in the blood in a zymogen form, and isconverted to an active form by either factor IXa, factor Xa, factor XIIa, orthrombin by minor proteolysis. Upon activation of the factor VII, a heavychain containing a catalytic domain and a light chain containing 2 EGF-likedomains are generated, and two chains are held together by a disulfide bond.In the presence of factor III and calcium ions, the activated factor thenfurther activates the coagulation cascade by converting factor IX to factorIXa and/or factor X to factor Xa. Alternative splicing of this gene resultsin 2 transcripts. Defects in this gene can cause coagulopathy. The expressionproduct is the active ingredient of Novoseven and Niastase.

Purity

>95%

Source

hamster kidney cells

Species

Human

Endotoxin

< 1.0 EU per μg of the protein

Protein Sequences

ANAFLEELRPGSLERECKEEQCSFEEAREIFKDAERTKLFWISYSDGDQCASSPCQNGGSCKDQLQSYICFCL
PAFEGRNCETHKDDQLICVNENGGCEQYCSDHTGTKRSCRCHEGYSLLADGVSCTPTVEYPCGKIPILEKRNA
SKPQGRIVGGKVCPKGECPWQVLLLVNGAQLCGGTLINTIWVVSAAHCFDKIKNWRNLIAVLGEHDLSEHDGDE
QSRRVAQVIIPSTYVPGTTNHDIALLRLHQPVVLTDHVVPLCLPERTFSERTLAFVRFSLVSGWGQLLDRGATALE
LMVLNVPRLMTQDCLQQSRKVGDSPNITEYMFCAGYSDGSKDSCKGDSGGPHATHYRGTWYLTGIVSWGQGC
ATVGHFGVYTRVSQYIEWLQKLMRSEPRPGVLLRAPFP

CAS number

9001-25-6

Storage

Can be stored at+4^oC short term (1-2 weeks). For long term storage, aliquot and store at-20^oC or -70^oC. Avoid repeated freezing and thawing cycles.

Antigen GeneInformation