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Active Recombinant Human RAF1, GST-tagged

Cat.No. : RAF1-551H
Product Overview : Recombinant Human RAF1(a.a. 306-648), fused with N-terminal GST-tag, was expressed in an Baculovirus infected Sf9 cell expression system.
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  • Gene Information
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Description : This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
Source : Sf9 insect cells
Species : Human
Tag : GST
Form : Aqueous buffer solution. 40 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 16 mM glutathione, 20% glycerol, 3 mM DTT.
Bio-activity : 158 pmol/min/µg.
Molecular Mass : 65 kDa
Protein length : 306–648
Purity : ≥80%
Applications : Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Storage : >6 months at –80°C. Avoid freeze/thaw cycles.
Concentration : 0.71 mg/ml
Gene Name : RAF1 Raf-1 proto-oncogene, serine/threonine kinase [ Homo sapiens (human) ]
Official Symbol : RAF1
Synonyms : RAF1; v-raf-1 murine leukemia viral oncogene homolog 1; NS5; CRAF; Raf-1; c-Raf; Oncogene RAF1; raf proto-oncogene serine/threonine protein kinase; EC 2.7.11.1; C-RAF; cRaf
Gene ID : 5894
mRNA Refseq : NM_002880
Protein Refseq : NP_002871
MIM : 164760
UniProt ID : P04049
Chromosome Location : 3p25
Pathway : Activation of NMDA receptor upon glutamate binding and postsynaptic events; Acute myeloid leukemia; Axon guidance; B cell receptor signaling pathway
Function : ATP binding; MAP kinase kinase kinase activity; identical protein binding; mitogen-activated protein kinase kinase binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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How is the RAF1 protein studied in a clinical context? 01/21/2023

The RAF1 protein is studied through a combination of molecular biology techniques, such as immunohistochemistry, Western blotting, and genetic sequencing, in both clinical and research settings.

Is RAF1 protein expression routinely tested in cancer diagnostics? 06/11/2019

RAF1 protein expression is not typically included in routine cancer diagnostics. Instead, mutations and expression levels of other genes, like BRAF, are often analyzed in cancer patients.

Are there any clinical trials focused on RAF1 protein for disease treatment or research? 01/14/2019

Clinical trials involving RAF1 protein are ongoing for conditions like Noonan syndrome and certain cancers. These trials aim to develop new therapies and better understand its role in disease.

How does the RAF1 protein influence cardiac health, and what clinical implications does this have? 02/28/2018

RAF1 mutations can affect cardiac development, leading to congenital heart defects and other cardiac issues in patients with Noonan syndrome. This has important clinical implications for cardiologists and genetic counselors.

Can RAF1 mutations be inherited, and if so, how does this affect patients and their families? 01/31/2016

Yes, RAF1 mutations can be inherited, and they can lead to Noonan syndrome. This can have a significant impact on affected individuals and their families, necessitating specialized medical care and genetic counseling.

Customer Reviews (3)

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Reviews
04/18/2020

    They understand that each research project may have unique requirements, and they are more than willing to address these specific needs.

    11/01/2017

      The manufacturer's commitment to providing excellent technical support is truly impressive.

      02/25/2016

        Their prompt and insightful responses to inquiries and troubleshooting requests enable me to tackle experimental obstacles with confidence and efficiency.

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