Recombinant Human TEK Tyrosine Kinase, Endothelial, His-tagged

• Cat.No.: TEK-547H
• Product Overview: Recombinant human TIE 2 was expressed bybaculovirus in Sf9 insect cellsusing an N-terminal GST tag. MW = 61 kDa.

Specification

• Cat. No.: TEK-547H
• Description: TIE 2 or TEK is a receptor tyrosine kinase that is expressed principally on vascular endothelium. Disrupting TIE 2 function in mice results in embryonic lethality with defects in embryonic vasculature, suggesting a role in blood vessel maturation and maintenance. Angiopoietin-1 is a secreted growth factor that binds to and activates the TIE 2 receptor tyrosine kinase. SHP2 and GRB2 are recruited to the activated TIE 2 kinase domain and are part of the cellular responses that mediate TIE 2 function. TIE 2 expression is upregulated in the endothelium of vascular "hot spots" in human breast cancer specimens. However, TIE 2 is also overexpressed in areas of active angiogenesis in normal tissues.
• Source: Baculovirus infected Sf9 cells.
• Purity: >60% by SDS-PAGE.
• Specific Activity: 140 U/ug. One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Tyr substarte per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Na-orthovanadate, 0.5 mM ATP, 30 ug/ml Poly (Glu:Tyr)4:1 substrate, and 2 ug/ml recombinant Tie2.
• Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Formulated In: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.
• Stability: >6 months at –80°C.

Gene Information

• Gene Name: TEK TEK tyrosine kinase, endothelial [ Homo sapiens ]
• Synonyms: TEK; TEK tyrosine kinase, endothelial; TIE2; VMCM; TIE-2; VMCM1; CD202B; soluble TIE2 variant 1; soluble TIE2 variant 2; p140 TEK
• Gene ID: 7010
• mRNA Refseq: NM_000459
• Protein Refseq: NP_000450
• MIM: 600221
• UniProt ID: Q02763
• Chromosome Location: 9p21
• Pathway: Hemostasis; Signaling in Immune system
• Function: ATP binding; nucleotide binding; protein binding; receptor activity; transferase activity; transmembrane receptor protein tyrosine kinase activity