| Cat. No. : |
Tslp-202M |
| Description : |
Thymic Stromal Lymphopoietin (TSLP) is a member of the B cell‐stimulating factor family. Originally identified from the conditioned medium of a mouse thymic stromal cell line that promoted B‐cell development, TSLP is expressed in thymus, spleen, kidney, lung, and bone marrow. TSLP activity overlaps with that of IL‐7, playing a key role in the development of B cells, as well as stimulating the growth of thymocytes and T cells. TSLP stimulates monocytes to produce T cell‐attracting chemokines such as TARC (CCL17) and MDC (CCL22). TSLP also activates dendritic cellprimed CD4 T cells to produce Th2 cytokines, including IL‐4, IL‐5, IL‐13, and TNFα. This hematopoietic cytokine binds to a heterodimeric receptor complex consisting of the IL‐7R alpha chain (IL‐7Rα) and the TSLP‐specific chain (TSLPR). Binding induces activation of STAT3 and STAT5 via phosphorylation. Mouse TSLP shares approximately 43% amino acid sequence identity with human TSLP. |
| Source : |
Insect cell. |
| Molecular Mass : |
15‐22 kDa on SDS‐PAGE due to glycosylation. |
| Purity : |
Greater than 99%, as determined by SDS‐PAGE. |
| Form : |
Sterile liquid; 20 mM NaH2PO4, 0.15M NaCl, pH 7.2 with 1.0% BSA. 0.22 μm filtered. |
| Endotoxin Level : |
Less than 0.1 ng/ug cytokine as determined by the LAL assay. |
| Bioactivity : |
The ED50 tested in a Nag8/7 cell proliferation assay was 0.15 ng/ml, corresponding to a specific activity of approximately 6x106 Units/mg. |
| Applications Tested : |
This recombinant mouse TSLP has been tested in a Nag8/7 cell proliferation assay with an observed ED50 of 0.15 ng/ml, corresponding to a specific activity of approximately 6x106 Units/mg. |
| Temperature Limitation : |
Store at less than or equal to ‐70°C. |
| Handling Conditions : |
For best recovery, quick‐spin vial prior to opening. Use in a sterile environment. |
