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Home > Products > Native Proteins > Native Human Amylase, Alpha 2B (pancreatic)

Native Human Amylase, Alpha 2B (pancreatic)

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Product Overview:
Native human AMY2B wasexpressed in Human Pancreas.
Amylases aresecreted proteins that hydrolyze 1,4-alpha-glucoside bonds inoligosaccharides and polysaccharides, and thus catalyze the first step indigestion of dietary starch and glycogen. The human genome has a cluster ofseveral amylase genes that are expressed at high levels in either salivarygland or pancreas. This gene encodes an amylase isoenzyme produced by thepancreas.
Aqueous buffer of100 mM Tris-HCL+150 mM NaCl.
Human Pancreas
Biological Activity:
Typically > 400Units/ml at 37 oC. One unit will catalyze thehydrolysis of one of malto pentaose, which through coupled reactions resultsin the formation of 5 of glucose-6-phosphate per minute at 37 oC.Measured at 340 nm as an equimolar amount of NADH produced by a coupledreaction.
> 95.0% asdetermined by RP-HPLC and SDS-PAGE analyses.
Liquid is stable for1 year at 2-4oC. Avoid repeated freeze-thaw cycles.
Carbohydrate digestion and absorption; Digestionof dietary carbohydrate; Metabolic pathways; Metabolism of carbohydrates; Pancreaticsecretion; Starch and sucrose metabolism

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

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