| Cat. No. : |
IGF1R-663H |
| Description : |
IGF1R,also known as somatomedin C, is the dominant effector of growth hormone andis structurally homologous to proinsulin. Human IGF1R is synthesized as twoprecursor isoforms with N- and alternate C- terminal propeptides. Theseisoforms are differentially expressed by various tissues. The 7.6 kDa matureIGF-I is identical between isoforms and is generated by proteolytic removalof the N- and C- terminal regions. Mature human IGF1R shares 94% and 96% aasequence identity with mouse and rat IGF-I, respectively, and exhibitscross-species activity. It shares 64% aa sequence identity with mature humanIGF-II. Circulating IGF-I is produced by hepatocytes, while local IGF-I isproduced by many other tissues in which it has paracrine effects. |
| Source : |
Human |
| Host : |
E. coli |
| Purity : |
>95%, asdetermined by SDS-PAGE and HPLC. |
| Sequence : |
MGKISSLPTQ LFKCCFCDFL KVKMHTMSSS HLFYLALCLL TFTSSATAGPETLCGAELVD ALQFVCGDRG FYFNKPTGYG SSSRRAPQTG IVDECCFRSC DLRRLEMYCA PLKPAKSARSVRAQRHTDMP KTQKEVHLKN ASRGSAGNKN YRM |
| Molecular Weight : |
Nativehuman Insulin Growth Factor-1 is generated by the proteolytic removal of thesignal peptide and propeptide, the molecule has a calculated molecular massof approximately 8 kDa. |
| Biological Activity : |
TheED50 was determined by a cell proliferation assay using FDC-P1 cells is ≤ 1.0ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg. |
| Endotoxin : |
Endotoxin contentwas assayed using a LAL gel clot method. Endotoxin level was found to be lessthan 0.1 ng/µg(1EU/µg). |
| Reconstitution : |
A quick spin of thevial followed by reconstitution in distilled water to a concentration notless than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage : |
The lyophilized protein is stable forat least 2 years from date of receipt at -20°C. Upon reconstitution, thiscytokine can be stored in working aliquots at 2°C - 8°C for one month, or at-20°C for six months, with a carrier protein without detectable loss ofactivity. Avoid repeated freeze/thaw cycles. |
| OfficialSymbol : |
IGF1R |
| Pathways : |
Adherens junction;Apoptosis; Endochondral Ossification; Endocytosis; Glioma; Glioma; IGF1pathway; Id Signaling Pathway; Insulin Signaling; Integrins in angiogenesis; Long-termdepression; Melanoma; Pathways in cancer; Pathways in cancer; Prostatecancer; Senescence and Autophagy |
