Recombinant Human Protein Tyrosine Phosphatase, Non-Receptor Type 1, GST-tagged

• Cat.No.: PTPN1-1908H
• Product Overview: RecombinantHuman PTPN1, full length, (aa 1-436) with N-terminal GST tag.

Specification

• Cat. No.: PTPN1-1908H
• Description: PTPN1 (PTP1B) isan enzyme that is the founding member of the protein tyrosine phosphatase(PTP) family. In humans it is encoded by the PTPN1 gene. PTPN1 is anegative regulator of the insulin signaling pathway and is considered apromising potential therapeutic target, in particular for treatment of type 2diabetes.
• Source: E. coli
• Species: Human
• Form: Liquid in 25 mM Tris-HCl, pH 8.0 +100 mM NaCl + 0.05% Tween-20 + 50% glycerol + 3 mM DTT.
• Molecular Weight: 76kDa
• Purity: > 70%
• Specific Activity: 10 U/ug.One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.Assay buffer: 50 mM HEPES, pH 7.4 + 2 mM EDTA + 3mM DTT + 100 mM NaCl + 50mM Pnpp.
• Applications: Useful for the study of enzymekinetics, regulation, to dephosphorylate target substrates and forscreening inhibitors.
• Storage: Stablefor 6 months at -80°C. Avoid repeated freeze-thaw cycles.
• Pathways: Adherensjunction; Calcineurin-regulated NFAT-dependent transcription in lymphocytes;Cytokine Signaling in Immune system; EGF receptor (ErbB1) signaling pathway;Growth hormone receptor signaling; Hemostasis; IGF1 pathway; Immune System;Insulin Pathway; Insulin signaling pathway; Integrin alphaIIb beta3signaling; Interferon alpha/beta signaling; N-cadherin signaling events;PDGFR-beta signaling pathway; Regulation of IFNA signaling

Gene Information

• Gene Name: PTPN1protein tyrosine phosphatase, non-receptor type 1 [ Homo sapiens ]
• Official Symbol: PTPN1
• Synonyms: PTPN1;protein tyrosine phosphatase, non-receptor type 1; PTP1B; tyrosine-proteinphosphatase non-receptor type 1; OTTHUMP00000031266; protein tyrosinephosphatase 1B; protein-tyrosine phosphatase 1B; protein tyrosine phosphatase,placental
• Gene ID: 5770
• mRNA Refseq: NM_002827
• Protein Refseq: NP_002818
• MIM: 176885
• UniProt ID: P18031
• Chromosome Location: 20q13.1-q13.2
• Function: ephrinreceptor binding; hydrolase activity; insulin receptor binding; proteinbinding; protein tyrosine phosphatase activity; zinc ion binding