Recombinant Human Mitogen-Activated Protein Kinase-Activated Protein Kinase 5, His-tagged

• Cat.No.: MAPKAPK5-1888H
• Product Overview: Recombinant humanfull length MAPKAPK5, His-tagged.

Specification

• Cat. No.: MAPKAPK5-1888H
• Description: MAPKAPK5 is anenzyme that in humans is encoded by the MAPKAPK5 gene. The protein encoded bythis gene is a member of the serine/threonine kinase family. In response tocellular stress and proinflammatory cytokines, this kinase is activatedthrough its phosphorylation by MAP kinases including MAPK1/ERK,MAPK14/p38-alpha, and MAPK11/p38-beta. In vitro, this kinase phosphorylatesheat shock protein HSP27 at its physiologically relevant sites. Twoalternately spliced transcript variants of this gene encoding distinctisoforms have been reported.
• Source: Insect cells
• Species: Human
• Form: Liquid in 50 mMTris, pH 7.5 + 150 mM NaCl + 0.05 mM EDTA + 0.05% Triton X-100 + 2 mM DTT +50% glycerol
• Molecular Weight: 58.3 kDa
• Concentration: 0.45 mg/ml
• Specific Activity: 220 nmole ofphosphate transferred to PRAK substrate peptide (KKLRRTLSVA) per minute permg of total protein at 30°C. Activity determined at a final proteinconcentration of 3.33 μg/ml.
• Storage: Stable for 6 monthsin working aliquots at -80°C. Avoid repeated freeze-thaw cycles.
• OfficialSymbol: MAPKAPK5

Gene Information

• Gene Name: MAPKAPK5 mitogen-activatedprotein kinase-activated protein kinase 5 [ Homo sapiens ]
• Synonyms: MAPKAPK5;mitogen-activated protein kinase-activated protein kinase 5; PRAK; MAPkinase-activated protein kinase 5; MAPKAPK-5; MAPKAP kinase 5;OTTHUMP00000241666; OTTHUMP00000241669; MAPK-activated protein kinase 5;p38-regulated/activated protein kinase; EC 2.7.11.1; EC 2.7.11
• Gene ID: 8550
• mRNA Refseq: NM_003668
• Protein Refseq: NP_003659
• MIM: 606723
• UniProt ID: Q8IW41
• Chromosome Location: 12q24.13
• Pathway: MAPK signalingpathway; Signaling mediated by p38-alpha and p38-beta; p38 MAPK SignalingPathway
• Function: ATP binding; MAPkinase kinase activity; nucleotide binding; protein binding; proteinserine/threonine kinase activity