| Cat. No. : |
IL6-5433M |
| Description : |
IL6 is a pleiotropic cytokine that plays animportant role in host defense by regulating immune and inflammatoryresponses. Produced by T cells, monocytes, fibroblasts, endothelial cells andkeratinocytes, IL6 has diverse biological functions. It stimulates B-celldifferentiation and antibody production, synergizes with IL-3 inmegakaryocyte development and platelet production, induces expression ofhepatic acute-phase proteins, and regulates bone metabolism. IL6 signalsthrough the IL6 receptor system that consists of two chains, IL6Rα and gp130. |
| Source : |
E. coli |
| Species : |
Macaca mulatta |
| Form : |
Lyophilized from a0.2 µm filtered concentrated solution in PBS, pH 7.4. |
| MolecularWeight : |
21.1 kDa |
| Purity : |
> 97% by SDS-PAGEand HPLC analyses |
| Endotoxin Level : |
< 0.1 ng/µg ofprotein |
| Biological Activity : |
The ED50as determined by the dose-dependent stimulation of the proliferation ofIL-6-dependent mouse 7TD1 cells is < 0.1 ng/ml, corresponding to aspecific activity of >1×107 units/mg. |
| Sequences : |
MAPVLPGEDSKNVAAPHSQP LTSSERIDKH IRYILDGISA LRKETCNRSN MCESSKEALA ENNLNLPKMA EKDGCFQSGFNEDTCLVKII TGLLEFEVYL EYLQNRFESS EEQARAVQMS TKVLIQFLQK KAKNLDAITT PEPTTNASLLTKLQAQNQWL QDMTTHLILR SFKEFLQSNL RALRQM |
| Reconstitution : |
Centrifuge vialprior to opening to bring contents to the bottom. Reconstitute in steriledistilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml.Further dilutions should be made in appropriate buffered solutions. |
| Storage : |
The lyophilizedproduct is stable for several weeks at 2-4°C, but should be stored desiccatedat -20°C. After reconstitution, the protein should be divided into workingaliquots and frozen at -80°C. Avoid repeated freeze-thaw cycles. |
| OfficialSymbol : |
IL6 |
