Recombinant Human 2,4-Dienoyl-CoA Reductase, Mitochondrial, His-tagged

• Cat.No.: DECR1-971H
• Product Overview: Recombinant human DECR1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques(35-335 aa).

Specification

• Cat. No.: DECR1-971H
• Description: DECR1 is a mitochondrial protein that exists as a homotetramer and belongs to the family of short-chain dehydrogenases/reductases. It functions as an auxiliary enzyme of beta-oxidation andt participates in the metabolism of unsaturated fatty enoyl-CoA esters. Specifically, DECR1 uses NADP+ to catalyze the reduction of 2,4-dienoyl-CoA to yield trans-3-enoyl-CoA, which can then be used as an intermediate in the Krebs cycle. Additionally, DECR1 is thought to function as a tumor suppressor, possibly downregulating the expression of Neu and slowing the rate of tumorigenesis.
• Form: Liquid. In 20mM Tris-HClbuffer(pH 8.0) containing 10% glycerol,1mM DTT.
• Molecular Weight: 34.4 kDa (322aa), confirmed by MALDI-TOF
• Purity: > 90% by SDS - PAGE
• Concentration: 1 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MNTEALQSKF FSPLQKAMLP PNSFQGKVAF ITGGGTGLGK GMTTLLSSLG AQCVIASRKM DVLKATAEQI SSQTGNKVHA IQCDVRDPDM VQNTVSELIK VAGHPNIVIN NAAGNFISPT ERLSPNAWKT ITDIVLNGTA FVTLEIGKQL IKAQKGAAFL SITTIYAETG SGFVVPSASA KAGVEAMSKS LAAEWGKYGM RFNVIQPGPI KTKGAFSRLD PTGTFEKEMI GRIPCGRLGT VEELANLAAF LCSDYASWIN GAVIKFDGGE EVLISGEFND LRKVTKEQWD TIEELIRKTK GS
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: DECR1 2,4-dienoyl CoA reductase 1, mitochondrial [Homo sapiens]
• Synonyms: DECR1; DECR; NADPH; SDR18C1; 2,4-dienoyl CoA reductase 1, mitochondrial; 2,4-dienoyl-CoA reductase, mitochondrial;4-enoyl-CoA reductase;short chain dehydrogenase/reductase family 18C, member 1; 4-enoyl-CoA reductase [NADPH]; EC1.3.1.34; 2,4-dienoyl-CoA reductase [NADPH]
• Gene ID: 1666
• mRNA Refseq: NM_001359
• Protein Refseq: NP_001350
• MIM: 222745
• UniProt ID: Q16698
• Chromosome Location: 8q21.3
• Function: 2,4-dienoyl-CoA reductase (NADPH) activity; binding; NADPH binding; 2,4-dienoyl-CoA reductase (NADPH) activity; oxidoreductase activity, acting on NADH or NADPH