Recombinant Human Aldolase B, Fructose-Bisphosphate, His-tagged

• Cat.No.: ALDOB-1918H
• Product Overview: Recombinant humanALDOB protein, fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography techniques.

Specification

• Cat. No.: ALDOB-1918H
• Description: ALDOB isone of three known aldolase isoenzymes, and is found in kidney and smalladult intestine where it is associated with aldolases A or C. ALDOB catalyzesthe reversible cleavage of fructose 1-phosphate into dihydroxyacetonephosphate and glyceraldehyde.
• Form: Liquid. In 20mMTris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl.
• Molecular Weight: 42 kDa(388aa) confirmed by MALDI-TOF
• Purity: > 95%by SDS-PAGE
• Concentration: 1 mg/ml(determined by Bradford assay)
• Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MGSHMAHRFP ALTQEQKKEL SEIAQSIVAN GKGILAADES VGTMGNRLQR IKVENTEENRRQFREILFSVDSSINQSIGGVILFHETLYQ KDSQGKLFRN ILKEKGIVVG IKLDQGGAPL AGTNKETTIQ GLDGLSERCA QYKKDGVDFGKWRAVLRIAD QCPSSLAIQE NANALARYAS ICQQNGLVPI VEPEVIPDGD HDLEHCQYVT EKVLAAVYKALNDHHVYLEG TLLKPNMVTA GHACTKKYTP EQVAMATVTALHRTVPAAVP GICFLSGGMS EEDATLNLNA INLCPLPKPW KLSFSYGRAL QASALAAWGG KAANKEATQEAFMKRAMANC QAAKGQYVHT GSSGAASTQS LFTACYTY
• Storage: Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: FOXA2 and FOXA3 transcription factornetworks; Fructose and mannose metabolism; Fructose catabolism;Gluconeogenesis; Glucose metabolism; Glycolysis; Glycolysis (Embden-Meyerhofpathway); Glycolysis / Gluconeogenesis; Glycolysis and Gluconeogenesis;Metabolic pathways; Metabolism of carbohydrates; Pentose phosphate pathway

Gene Information

• Gene Name: ALDOB aldolase B,fructose-bisphosphate [ Homo sapiens ]
• Official Symbol: ALDOB
• Synonyms: ALDOB; aldolase B, fructose-bisphosphate; ALDB;ALDO2; fructose-bisphosphate aldolase B; aldolase 2; liver-type aldolase;aldolase B, fructose-bisphosphatase; EC 4.1.2.13
• Gene ID: 229
• mRNA Refseq: NM_000035
• Protein Refseq: NP_000026
• MIM: 612724
• UniProt ID: P05062
• Chromosome Location: 9q21.3-q22.2
• Function: ATPase binding; cytoskeletal proteinbinding; fructose binding; fructose-bisphosphate aldolase activity; identicalprotein binding; lyase activity; phosphatidylcholine binding; protein binding