Recombinant Human Osteoprotegerin Ligand

• Cat.No.: TNFSF11-P1002H
• Product Overview: Recombinanthuman OPGL fused with Fc fragment of Human IgG or 6His was produced in CHOcells.

Specification

• Cat. No.: TNFSF11-P1002H
• Description: Thetumour-necrosis-factor-family molecule osteoprotegerin ligand has beenidentified as a potential osteoclast differentiation factor and regulator ofinteractions between T cells and dendritic cells in vitro. OPGL is a newregulator of lymph-node organogenesis and lymphocyte development and is anessential osteoclast differentiation factor in vivo. Osteoblasts/stromalcells express OPGL /TNF-related activation-induced cytokine /receptoractivator of NF-kB ligand (RANKL)" as a membrane associated factor.Osteoclast precursors which possess RANK, a TNF receptor family member,recognize OPGL/TRANCE/RANKL through cell-to-cell interaction withosteoblasts/stromal cells, and differentiate into osteoclasts in the presenceof macrophage colony-stimulating factor.
• ProteinConstruction: Recombinanthuman OPGL is combined the Sequence of extracellular region of OPGL and Fcfragment of Human IgG or 6His.
• Source: ChineseHamster Ovary (CHO cell)
• Purity: >95%as determined by SDS-PAGE and HPLC
• Endotoxin: <1.0EU per 10 mg protein as determined by the LAL method
• Stability: Samplesare stable for up to six months from date of receipt at -20℃ and are stable fortwo months at 4℃.
• Molecular Mass: Recombinant humanOPGL comprises 410 and 184 amino acids of OPGL-Fc and OPGL-6His,respectively. It is about 80kDa and 33 kDa of OPGL-Fc and OPGL-6His, underreducing conditions, respectively.
• Predicted N terminal: IRAEKAMVDG
• Formulation: Normally20-50mM NaAC-HAC and 100-150 mM NaCl are added, pH 5.0-6.0.
• Storage: Store itunder sterile conditions at -70℃upon receiving. Recommend to pack the protein intosmaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: TNFSF11 tumor necrosis factor (ligand) superfamily, member11 [ Homo sapiens ]
• Official Symbol: TNFSF11
• Synonyms: TNFSF11; tumor necrosis factor (ligand) superfamily, member 11;ODF; OPGL; sOdf; CD254; OPTB2; RANKL; TRANCE; hRANKL2; tumor necrosis factorligand superfamily, member 11; OTTHUMP00000178585; osteoprotegerin ligand;osteoclast differentiation factor; TNF-related activation-induced cytokine;receptor activator of nuclear factor kappa B ligand; Tumor necrosis factorligand superfamily member 11, membrane form; Tumor necrosis factor ligandsuperfamily member 11, soluble form; CD254 antigen; Osteoprotegerin ligand
• Gene ID: 8600
• mRNA Refseq: NM_003701
• Protein Refseq: NP_003692
• MIM: 602642
• UniProt ID: O14788
• Chromosome Location: 13q14
• Pathway: Cytokine-cytokine receptor interaction; IL6-mediated signaling events; Osteoblast Signaling
• Function: cytokineactivity; protein binding; receptor activity; tumor necrosis factor receptorbinding