Active Recombinant Human SPAM1 / PH20 / Hyaluronidase protein, Tag Free

• Cat.No.: SPAM1-P1004H
• Product Overview: Active Recombinant Human SPAM1 / PH20 / Hyaluronidase protein was expressed in CHO.

Specification

• Source: CHO
• Species: Human
• Tag: N/A
• Form: Liquid in sterile 20mM PB, 300mM NaCl, 0.1mM EDTA, 0.1mM CaCl2, pH6.8.
• Bio-activity: The activity of Human Hyaluronidase is measured by its ability to hydrolyze HA in turbidimetric assay. The specific activity is 53,200 IU/mg. (Unit definition: One unit will cause a change in A600nm of 0.330 per minute at pH 5.7 at 37 ºC.)
• Molecular Mass: The protein has a calculated MW of 51 kDa. The protein migrates as 60 kDa under reducing condition (SDS-PAGE) due to glycosylation.
• AA Sequence: LNFRAPPVIPNVPFLWAWNAPSEFC LGKFDEPLDMSLFSFIGSPRINATG QGVTIFYVDRLGYYPYIDSITGVTV NGGIPQKISLQDHLDKAKKDITFYM PVDNLGMAVIDWEEWRPTWARNWKP KDVYKNRSIELVQQQNVQLSLTEAT EKAKQEFEKAGKDFLVETIKLGKLL RPNHLWGYYLFPDCYNHHYKKPGYN GSCFNVEIKRNDDLSWLWNESTALY PSIYLNTQQSPVAATLYVRNRVREA IRVSKIPDAKSPLPVFAYTRIVFTD QVLKFLSQDELVYTFGETVALGASG IVIWGTLSIMRSMKSCLLLDNYMET ILNPYIINVTLAAKMCSQVLCQEQG VCIRKNWNSSDYLHLNPDNFAIQLE KGGKFTVRGKPTLEDLEQFSEKFYC SCYSTLSCKEKADVKDTDAVDVCIA DGVCIDAFLKPPMETEEPQIFY
• Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.
• Purity: > 95% as determined by SDS-PAGE. > 95% as determined by SEC-HPLC.
• Storage: Store it under sterile conditions at -20 to -80 ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Concentration: 0.5mg/ml.

Gene Information

• Gene Name: SPAM1 sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) [ Homo sapiens ]
• Official Symbol: SPAM1
• Synonyms: SPAM1; HYA1; PH20; HYAL1; HYAL3; HYAL5; PH-20; SPAG15; MGC26532; hyaluronidase PH-20; hyal-PH20; OTTHUMP00000211901; OTTHUMP00000211902; OTTHUMP00000211952; sperm surface protein PH-20; hyaluronoglucosaminidase PH-20
• Gene ID: 6677
• mRNA Refseq: NM_001174044
• Protein Refseq: NP_001167515
• MIM: 600930
• UniProt ID: P38567