Active Recombinant Human Thrombospondin 4, His-tagged

• Cat.No.: THBS4-507H
• Product Overview: Recombinant Human THBS4protein was expressed in CHO with a C-terminal 10-His tag. Ala22-Asn961.

Specification

• Cat. No.: THBS4-507H
• Description: THBS4 is a 140 kDacalcium-binding protein that interacts with other extracellular matrixmolecules and modulates the activity of various cell types. TSP1 and 2constitute subgroup A and form disulfide-linked homotrimers. The human THBS4cDNA encodes a 961 amino acid precursor that includes a 26 aa signal sequencefollowed by an N-terminal heparin-binding domain, a coiled-coil motif, fourEGF-like repeats, seven TSP type3 repeats, and a TSP C-terminal domain.
• Source: Chinese HamsterOvary cell line, CHO-derived.
• Form: Lyophilized from a0.2 μm filtered solution in Tris, NaCl and CaCl2.
• N-terminalSequence: Ala22
• Molecular Weight: 105 kDa
• Activity: Measuredby the ability of the immobilized protein to support the adhesion of SVEC410mouse endothelial cells. rh THBS4 is coated to 96 well plates at 10 μg/mL(100 μL/well) overnight then reduced with 20 mM DTT for 30 minutes. When 2 x104 cells/ well are added to rh THBS4 coated plates, approximately50-70% will adhere after one hour at 37°C.
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• SDS-PAGE: 145 kDa,reducing conditions.
• Purity: >90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 100 μg/ mLin sterile PBS
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months fromdate of receipt, -20 to -70°C as supplied. 1 month, 2 to 8 °C under sterileconditions after reconstitution. 3 months, -20 to -70°C under sterileconditions after reconstitution.

Gene Information

• Gene Name: THBS4 thrombospondin 4 [ Homosapiens ]
• Official Symbol: THBS4
• Synonyms: THBS4; thrombospondin4; TSP4; thrombospondin-4; OTTHUMP00000135313; OTTHUMP00000222343
• Gene ID: 7060
• mRNA Refseq: NM_003248
• Protein Refseq: NP_003239
• MIM: 600715
• UniProt ID: P35443
• Chromosome Location: 5q13
• Pathway: ECM-receptor interaction; Focal Adhesion;Focal adhesion; Malaria; Phagosome; Phagosome; Signal Transduction; Signalingby PDGF; TGF-beta signaling pathway
• Function: calcium ion binding; collagen binding;fibronectin binding; heparin binding; integrin binding; laminin-1 binding;structural molecule activity