Active Recombinant Rat Unc-5 Homolog A (C. elegans), Fc-tagged

• Cat.No.: Unc5a-513R
• Product Overview: Recombinant Rat Unc5aprotein was expressed in Murine myeloma cell line with the Fc part of HumanIgG1 Pro100-Lys330 (C-terminus) and Gln26-Asp358 (N-terminus).

Specification

• Cat. No.: Unc5a-513R
• Description: Unc5a is a member ofthe UNC5 family of type I transmembrane proteins within the immunoglobulinsuperfamily. UNC5H14 have two Ig and one or two thrombospondin type 1 domainin their extracellular regions, and a ZU5 domain, a DCC binding domain and aC-terminal death domain in their cytoplasmic regions. Rat Unc5a cDNA encodes898 amino acids including a 25 aa signal peptide, a 336 aa extracellulardomain with two TSP1 domains, and a 516 aa cytoplasmic domain.
• Source: Mammalian cells
• Form: Lyophilized from a0.2 μm filtered solution in PBS.
• N-terminalSequence: Gln26 predicted
• Molecular Weight: 63.4 kDa
• Activity: Measuredby its binding ability in a functional ELISA. Immobilized rr Unc5a/Fc Chimera at 5 μg/ mL (100 μL/ well) can bind rchNetrin-1with a linear range of 6-400 ng/mL.
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• SDS-PAGE: 85-95kDa & 40-43 kDa, reducing conditions.
• Purity: >90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 100 μg/ mLin sterile PBS
• Storage: Use a manualdefrost freezer and avoid repeated freeze-thaw cycles. 12 months from date ofreceipt, -20 to -70°C as supplied. 1 month, 2 to 8 °C under sterile conditionsafter reconstitution. 3 months, -20 to -70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: Unc5a unc-5 homolog A (C.elegans) [ Rattus norvegicus ]
• Official Symbol: Unc5a
• Synonyms: Unc5a; unc-5homolog A (C. elegans); Unc5h1; netrin receptor UNC5A; unc-5 homolog 1;protein unc-5 homolog 1; protein unc-5 homolog A; transmembrane receptorUnc5H1
• Gene ID: 60629
• mRNA Refseq: NM_022206
• Protein Refseq: NP_071542
• UniProt ID: O08721
• Chromosome Location: 17p14
• Pathway: Apoptosis; Axonguidance; Developmental Biology; Netrin mediated repulsion signals; Netrin-1signaling; Regulation of Apoptosis; Role of DCC in regulating apoptosis
• Function: netrin receptoractivity; receptor activity