Active Recombinant Human N-Acylsphingosine Amidohydrolase (non-lysosomal ceramidase) 2, His-tagged

• Cat.No.: ASAH2-582H
• Product Overview: Recombinant Human ASAH2protein was expressed in Chinese Hamster Ovary cell line with a N-terminal 6-Histag. Thr15-Ile761.

Specification

• Cat. No.: ASAH2-582H
• Description: The human ASAH2 geneencodes Nacylsphingosine amidohydrolase2, also known as neutral ornonlysosomal ceramidase. ASAH2 is a type II integral membrane protein thatcan be cleaved to produce a soluble secreted protein. The enzyme is abundantin the brush border membranes of the intestine, but is also expressed intissues such as kidney, brain and liver. An Nterminally truncated form ofhuman ASAH2 has been shown to localize to mitochondria.
• Source: Chinese HamsterOvary cell line, CHO-derived.
• Form: Supplied as a 0.2 μmfiltered solution in MES, NaCl and Sodium Cholate.
• N-terminalSequence: His
• Molecular Weight: 83 kDa
• Activity: Measured by itsability to hydrolyze the substrate C12:0 ceramide into sphingosine anddodecanoic acid. The specific activity is > 5,000 pmoles/min/μg, asmeasured under the described conditions.
• Endotoxin Level: < 1.0EU per 1 μg of the protein by the LAL method.
• SDS-PAGE: 132 kDa,reducing conditions.
• Purity: > 95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Use a manual defrostfreezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt,-20 to -70°C as supplied. 3 months, -20 to -70°C under sterile conditionsafter opening.

Gene Information

• Gene Name: ASAH2 N-acylsphingosineamidohydrolase (non-lysosomal ceramidase) 2 [ Homo sapiens ]
• Official Symbol: ASAH2
• Synonyms: ASAH2;N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2; HNAC1; BCDase;LCDase; NCDase; N-Cdase; neutral ceramidase; mitochondrial ceramidase;non-lysosomal ceramidase; acylsphingosine deacylase 2; neutral/alkalineceramidase; N-acylsphingosine amidohydrolase 2; EC 3.5.1.23
• Gene ID: 56624
• mRNA Refseq: NM_001143974
• Protein Refseq: NP_001137446
• MIM: 611202
• UniProt ID: Q9NR71
• Chromosome Location: 10q11.23
• Pathway: Glycosphingolipidmetabolism; Metabolic pathways; Metabolism; Metabolism of lipids andlipoproteins; Sphingolipid metabolism; Sphingosine biosynthesis; sphingolipidmetabolism
• Function: ceramidase activity;hydrolase activity