Catalog # or name ...
RecombinantMouse Gzmd protein was expressed in Murine myeloma cell line. Ile21-Leu252,with a C-terminal 10-His tag.
Gzmd is amember of the granzyme family of the serine proteases found specifically inthe cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer(NK) cells. Gzmd’sfunctions are largely unknown. However, its selective expression in primaryand functional hematopoietic stromal lines correlates with capacity ofsupporting growth of a pre-T lymphoma clone. Together with Granzymes E, F andG, it is regulated through pregnancy and by IL-2 and IL-15 in granulatedmetrial gland cells. The human or rat counterpart of mouse Gzmd has notbeen found. Like other granzymes, mouse Gzmd is notsecreted as a zymogen but stored as a fully processed and activated enzyme inthe cytoplasmic granules of CTL. It is synthesized as a 252 amino acidprecursor with an 18 amino acid signal peptide and a 2 amino acid propeptide.The mature protein (residues 21-252) is expressed and purified.
Murine myeloma cellline, NS0-derived.
Supplied as a 0.2 umfiltered solution in HEPES and NaCl.
Ile21 & His25
Measuredby its ability to cleave a peptide substrate, Succinyl-Phe-Leu-Phe-ThioBenzylester (Suc-FLF-SBzl), in the presence of 5, 5’-Dithiobis (2-nitrobenzoic acid)(DTNB). The specific activity is > 50 pmoles/min/μg, as measured under thedescribed conditions.
<1.0EU per 1μg of the protein by the LAL method.
42 kDa,reducing conditions.
>95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstituteat 100μg/mL in sterile 25 mM HEPES and 200 mM NaCl, pH 7.5.
Use amanual defrost freezer and avoid repeated freezethaw cycles. 6 monthsfrom date of receipt, -20 to -70oC as supplied. 3 months, -20 to -70oC understerile conditions after opening.
Gzmd granzyme D [ Musmusculus ]
Gzmd; granzyme D; CCP5;Ctla5; Ctla-5
Activation;Apoptosis; Intrinsic Pathway for Apoptosis
hydrolase activity;peptidase activity; serine-type peptidase activity
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