Recombinant Mouse Granzyme D, His-tagged

Cat. No.

Gzmd-1967M

Product Overview

RecombinantMouse Gzmd protein was expressed in Murine myeloma cell line. Ile21-Leu252,with a C-terminal 10-His tag.

Description

Gzmd is amember of the granzyme family of the serine proteases found specifically inthe cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer(NK) cells. Gzmd’sfunctions are largely unknown. However, its selective expression in primaryand functional hematopoietic stromal lines correlates with capacity ofsupporting growth of a pre-T lymphoma clone. Together with Granzymes E, F andG, it is regulated through pregnancy and by IL-2 and IL-15 in granulatedmetrial gland cells. The human or rat counterpart of mouse Gzmd has notbeen found. Like other granzymes, mouse Gzmd is notsecreted as a zymogen but stored as a fully processed and activated enzyme inthe cytoplasmic granules of CTL. It is synthesized as a 252 amino acidprecursor with an 18 amino acid signal peptide and a 2 amino acid propeptide.The mature protein (residues 21-252) is expressed and purified.

Source

Murine myeloma cellline, NS0-derived.

Form

Supplied as a 0.2 umfiltered solution in HEPES and NaCl.

N-terminalSequence

Ile21 & His25

MolecularWeight

27 kDa

Activity

Measuredby its ability to cleave a peptide substrate, Succinyl-Phe-Leu-Phe-ThioBenzylester (Suc-FLF-SBzl), in the presence of 5, 5’-Dithiobis (2-nitrobenzoic acid)(DTNB). The specific activity is > 50 pmoles/min/μg, as measured under thedescribed conditions.

Endotoxin Level

<1.0EU per 1μg of the protein by the LAL method.

SDS-PAGE

42 kDa,reducing conditions.

Purity

>95%,by SDS-PAGE under reducing conditions and visualized by silver stain.

Reconstitution

Reconstituteat 100μg/mL in sterile 25 mM HEPES and 200 mM NaCl, pH 7.5.

Storage

Use amanual defrost freezer and avoid repeated freezethaw cycles. 6 monthsfrom date of receipt, -20 to -70^oC as supplied. 3 months, -20 to -70^oC understerile conditions after opening.

Gene Information