| Cat. No. : |
Gpc2-5625M |
| Description : |
Theglypicans (GPC) constitute a family of heparan sulfate proteoglycans that areattached to the cell surface by a glycosylphosphatidylinositol (GPI) anchor.Six members of this family have been identified in mammals (GPC1-GPC6). Allglypican core proteins contain an N-terminal signal peptide, a large globularcysteine-rich domain (CRD) with 14 invariant cysteine residues, a stalk-likeregion containing the heparan sulfate attachment sites, and a C-terminal GPIattachment site. Based on the degree of their amino acid sequence similarity,two subfamilies of glypicans have been defined. |
| Source : |
Mammalian cells |
| Form : |
Lyophilized from a0.2 um filtered solution in Tris-Citrate and NaCl. |
| N-terminalSequence : |
Lys27 & Ala359 |
| MolecularWeight : |
58.9 kDa |
| Activity : |
Measuredby its binding ability in a functional ELISA. Immobilized rm Gpc2 at 4μg/mL(100μL/well) can bind rhMidkine with a linear range of 4-200 ng/mL. |
| Endotoxin Level : |
<1.0EU per 1μg of the protein by the LAL method. |
| SDS-PAGE : |
60 kDa, non-reducingconditions. |
| Purity : |
>95%,by SDS-PAGE under reducing conditions and visualized by silver stain. |
| Reconstitution : |
Reconstituteat 100μg/mL in PBS. |
| Storage : |
Use a manual defrost freezer and avoid repeated freezethawcycles. 12months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °Cunder sterile conditions after reconstitution. 3 months, -20 to -70 °C understerile conditions after reconstitution. |
