Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
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Recombinant Mouse Fibroblast Growth Factor 7

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Cat. No.:
Fgf7-530M
Product Overview:
Recombinant Mouse Fgf7protein was expressed in E. coli. Cys32-Thr194.
Description:
KGF, also known as Fgf7,is one of 22 known members of the mouse FGF family of secreted proteins that playsa key role in development, morphogenesis, angiogenesis, wound healing, and tumorigenesis.Fgf7 expression is restricted to cells of mesenchymal origin. When secreted, itacts as a paracrine growth factor for nearby epithelial cells. Fgf7 speeds woundhealing by being dramatically upregulated in response to damage to skin or internalstructures that results in high local concentrations of inflammatory mediatorssuch as IL1 and TNFα.
Source:
E. coli-derived
Form:
Lyophilized from a 0.2μm filtered solution in MOPS, Na2SO4, EDTA and DTT withBSA as a carrier protein.
N-terminalSequence:
Cys32
Molecular Weight:
18.7 kDa
Activity:
Measuredin a cell proliferation assay using 4MBr5 monkey epithelial cells. Rubin, J.S.et al. (1989) Proc. Natl. Acad. Sci. USA 86:802. The ED50 for thiseffect is typically 10-50 ng/mL.
Endotoxin Level:
<1.0 EUper 1 μg of the protein by the LAL method.
SDS-PAGE:
19 kDa,reducing conditions
Purity:
>90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution:
Reconstituteat 250 μg/mL in sterile PBS that containing at least 0.1% human or bovine serumalbumin.
Storage:
Use a manualdefrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt,-20 to -70°C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70°C under sterile conditions after reconstitution.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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