Active Recombinant Mouse Colony Stimulating Factor 3 (granulocyte), His-tagged

• Cat.No.: Csf3-5616M
• Product Overview: Recombinant Mouse Csf3protein was expressed in Murine myeloma cell line with aC-terminal 6-His tag. Met1-Asp626.

Specification

• Cat. No.: Csf3-5616M
• Description: Csf3 is apleiotropic cytokine best known for its specific effects on theproliferation, differentiation, and activation of hematopoietic cells of theneutrophilic and granulocyte lineage. Csf3 plays an important role in defenseagainst infection, in inflammation and repair, and in the maintenance ofsteady state hematopoiesis. Cell activation by Csf3 is mediated bygranulocyte colony stimulating factor receptor alpha, a 95 105 kDa type Itransmembrane protein and member of the cytokine receptor superfamily, type Icytokine receptor family, and type 2 subfamily of receptor proteins.
• Source: Mammalian cells
• Form: Lyophilized from a0.2 μm filtered solution in PBS with Trehalose.
• N-terminalSequence: Cys26
• Molecular Weight: 68.2 kDa
• Activity: Measuredby its ability to inhibit the Csf3-induced proliferation of NFS-60 mousemyeloid cells. The ED50 for this effect is typically 0.02-0.12μg/mL in the presence of 0.125 ng/mL of recombinant mouse Csf3.
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• SDS-PAGE: 100-110kDa, reducing conditions
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstituteat 100 μg/mL in PBS
• Storage: Use a manualdefrost freezer and avoid repeated freeze-thaw cycles. 12 months from date ofreceipt, -20 to -70°C as supplied. 1 month, 2 to 8 °C under sterile conditionsafter reconstitution. 3 months, -20 to -70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: Csf3 colony stimulating factor3 (granulocyte) [ Mus musculus ]
• Official Symbol: Csf3
• Synonyms: Csf3; colonystimulating factor 3 (granulocyte); Csfg; G-CSF; MGI-IG; granulocytecolony-stimulating factor
• Gene ID: 12985
• mRNA Refseq: NM_009971
• Protein Refseq: NP_034101
• UniProt ID: P09920
• Chromosome Location: 11 D; 11 57.0 cM
• Pathway: Cytokine-cytokinereceptor interaction; Cytokines and Inflammatory Response (BioCarta);Hematopoietic cell lineage; Jak-STAT signaling pathway; Malaria
• Function: cytokine activity;enzyme binding; growth factor activity