Recombinant Mouse Chemokine (C-C motif) Ligand 9

Cat. No.

Ccl9-403M

Product Overview

Recombinantmouse Ccl9 protein was expressed in E. coli. Gln22-Gln122.

Description

Mouse Ccl9 (alsoMIP1γ and MRP2) is an 11 kDa, secreted, monomeric polypeptide that belongs tothe β (or CC) intercrine family of chemokines. Based on its activity andamino acid (aa) sequence, it is further classified as a member of the NC6 orsixcysteine containing CC subfamily of chemokines.

Source

E. coli

Form

Lyophilized from a0.2 μm filtered solution in Acetonitrile and TAF with BSA as a carrierprotein.

N-terminal Sequence

Gln22

Molecular Weight

11.5 kDa

Activity

Measured by itsability to chemoattract THP-1 human acute monocytic leukemia cells. The ED50for this effect is typically 0.2-0.6 μg/mL. Measured by its ability tochemoattract human CCR1 transfected BaF3 mouse pro-B cells. The ED50for this effect is typically 0.002-0.01 μg/mL.

Endotoxin Level

<1.0 EU per 1 μgof the protein by the LAL method.

Purity

>97%,by SDS-PAGE under reducing conditions and visualized by silver stain.

Reconstitution

Reconstitute at 25μg/mL in sterile PBS that containing at least 0.1% human or bovine serumalbumin.

Storage

Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70^oC as supplied. 1month, 2 to 8^oC under sterile conditions after reconstitution. 3 months, -20 to-70^oC under sterile conditions after reconstitution.

Gene Information