Recombinant Human UBE2N, His-tagged

• Cat.No.: UBE2N-62H
• Product Overview: Recombinant human UBE2N protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Specification

• Cat. No.: UBE2N-62H
• Description: UBE2N, also known as UBC13, is a member of the E2 ubiquitin-conjugating enzyme family. It catalyzes the ATP-dependent synthesis of non-canonical polyubiquitin chains, a process that does not lead to proteasomal degradation. It mediates the transcription of several target genes and is thought to play a role in cell cycle progression; cellular differentiation and DNA repair mechanisms that ensure cell survival after DNA damage.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT
• Molecular Weight: 19.3 kDa(172aa), confirmed by MALDI-TOF
• Purity: > 95% by SDS - PAGE
• Concentration: 0.5 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MAGLPRRIIK ETQRLLAEPV PGIKAEPDES NARYFHVVIA GPQDSPFEGG TFKLELFLPE EYPMAAPKVRFMTKIYHPNV DKLGRICLDI LKDKWSPALQ IRTVLLSIQA LLSAPNPDDP LANDVAEQWK TNEAQAIETA RAWTRLYAMN NI
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: UBE2N ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast) [ Homo sapiens ]
• Synonyms: ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast); UBC13; MGC8489; UbcH-ben; MGC131857; UBE2N; ubiquitin-conjugating enzyme E2 N; yeast UBC13 homolog; ubiquitin-protein ligase N; ubiquitin carrier protein N; bendless-like ubiquitin conjugating enzyme; ubiquitin-conjugating enzyme E2N (homologous to yeast UBC13); bendless-like ubiquitin-conjugating enzyme; EC=6.3.2.19
• Gene ID: 7334
• mRNA Refseq: NM_003348
• Protein Refseq: NP_003339
• MIM: 603679
• UniProt ID: P61088
• Chromosome Location: 12q22
• Function: ATP binding; ligase activity; nucleotide binding; protein binding; ubiquitin binding; ubiquitin-protein ligase activity