| Cat. No. : |
Fcgrt-491M |
| Description : |
Fc receptor (FCRN) isan approximately 45 kDa transmembrane glycoprotein with structural homology toMHC class I proteins. It is widely expressed in endothelial and epithelial cellsand plays an important role in IgG homeostasis. It does not bind the structurallyrelated chicken IgY. FCRN additionally binds to albumin, and both it and IgG arebound at pH 5.0 but not at pH 8.0. FCRN associates noncovalently with beta 2Microglobulin,and this interaction is important for the intracellular trafficking of FCRN. FCRNcycles between the plasma membrane and acidified intracellular compartments ofendothelial cells and epithelial cells. It binds endocytosed IgG and albumin inthe low pH vesicles and transports them to the plasma membrane for extracellularrelease at higher Ph. FCRN is also expressed in neutrophils and myeloid antigenpresenting cells. It can enhance IgGmediated phagocytosis and antigen presentationby these cells, but it promotes the degradation of opsonizing IgG rather thanreturning it to the circulation. |
| Source : |
Mammalian cells |
| Form : |
Lyophilized from a 0.2μm filtered solution in PBS, NaCl and Triton. |
| N-terminal Sequence : |
Ser22 |
| Molecular Weight : |
32.3 kDa |
| Activity : |
Measured by its bindingability in a functional ELISA. When Recombinant Mouse FCRN is immobilized at 0.5μg/mL (100 μL/well), the concentration of human IgG that produces 50% of the optimalbinding response is found to be approximately 2 10 μg/mL. |
| Purity : |
>95%, by SDS-PAGEunder reducing conditions and visualized by silver stain. |
| Endotoxin Level : |
<1.0 EU per 1 μg of the proteinby the LAL method. |
| Reconstitution : |
Reconstitute at 250 μg/mL in PBS. |
| Storage : |
Use a manual defrostfreezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| OfficialSymbol : |
Fcgrt |
