Active Recombinant Rat Chemokine (C-X-C motif) Ligand 5

• Cat.No.: Cxcl5-445R
• Product Overview: RecombinantRat Cxcl5 protein was expressed in E. coli. Ala38-Arg125& Ala38-Lys117.

Specification

• Cat. No.: Cxcl5-445R
• Description: Cxcl5 is a chemokine originallycloned from LPS stimulated mouse fibroblasts. Rat Cxcl5 sharesapproximately 74% amino acid (aa) sequence identity with mouse Cxcl5 and islikely the rat orthologue to mouse Cxcl5. Rat Cxcl5 cDNAencodes a 130 aa residue precursor with a predicted 37 aa residue signalpeptide and a 93 aa residue mature protein. Among human CXC chemokines, rat Cxcl5 is mostclosely related to human GCP2 and ENA78. Rat Cxcl5 alsodiffers from these two human proteins by having an extended carboxyterminus.
• Source: E. coli
• Form: Lyophilized from a0.2 um filtered solution in Acetonitrile and TFA with BSA as a carrierprotein.
• N-terminalSequence: Ala38
• MolecularWeight: 10.1 kDa
• Activity: Measuredby its ability to chemoattract human CXCR2 transfected BaF3 mouse proB cells.The ED50 for this effect is typically 0.03 0.15 μg/mL.
• Endotoxin Level: <1.0EU per 1 μg of the protein by the LAL method.
• Purity: >97%,by SDSPAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstituteat 100μg/mL insterile PBS containing at least 0.1% human or bovine serum albumin.
• Storage: Use amanual defrost freezer and avoid repeated freezethaw cycles. 12 months from date ofreceipt, 20 to 70°C as supplied. 1 month, 2 to 8°C under sterileconditions after reconstitution. 3 months, 20 to 70°C under sterileconditions after reconstitution.

Gene Information

• Gene Name: Cxcl5 chemokine (C-X-C motif)ligand 5 [ Rattus norvegicus ]
• Official Symbol: Cxcl5
• Synonyms: Cxcl5; chemokine(C-X-C motif) ligand 5; Cxcl6; C-X-C motif chemokine 5; cytokineLIX; CXC chemokine LIX; small-inducible cytokine B5; chemokine (C-X-C motif)ligand 6 (granulocyte chemotactic protein 2)
• Gene ID: 60665
• mRNA Refseq: NM_022214
• Protein Refseq: NP_071550
• UniProt ID: P97885
• Chromosome Location: 14
• Pathway: Chemokine receptorsbind chemokines; Chemokine signaling pathway; Class A/1 (Rhodopsin-likereceptors); G alpha (i) signalling events; GPCR downstream signaling; GPCRligand binding; Peptide ligand-binding receptors; Pertussis; Rheumatoidarthritis; Signal Transduction; Signaling by GPCR
• Function: chemokine activity