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Active Recombinant Mouse Adhesion Molecule, Interacts With CXADR Antigen 1

Cat.No. : Amica1-447M
Product Overview : Recombinant Mouse Amica1protein was expressed in Murine myeloma cell line. Leu23-Leu281.
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Cat. No. : Amica1-447M
Description : Amica1 is a 65kDa, type I transmembrane glycoprotein that belongs to the junctionaladhesion molecule (JAM) subset of the immunoglobulin superfamily. JAM familyproteins contribute to intercellular connections within epithelial andendothelial cell layers and mediate their interactions with varioushemopoietic cells. The mouse Amica1 cDNA encodes a 379amino acid (aa) precursor that includes a 20 aa signal sequence, a 261 aaextracellular domain (ECD) with two Iglike domains, a 21 aa transmembranesegment, and a 77 aa cytoplasmic domain. In contrast to other JAM familyproteins, AMICA does not contain a cytoplasmic PDZ binding motif.
Source : Mammalian cells
Form : Lyophilized from a0.2 um filtered solution in PBS.
N-terminalSequence : Leu23
MolecularWeight : 56.6 kDa (monomer)
Activity : Measuredby the ability of the immobilized protein to support the adhesion of human neutrophils.When 2 x 105 cells/well are added to rmAMICAcoated plates (10 μg/mL, 100μL/well), 30 50% of the cells will adhere after 20 minutes at 37°C.
Endotoxin Level : <1.0EU per 1μg of the protein by the LAL method.
Purity : >95%,by SDSPAGE under reducing conditions and visualized by silver stain.
Reconstitution : Reconstitute at 100μg/mLin sterile PBS.
Storage : Use amanual defrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt, 20 to 70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3months, 20 to 70°C under sterile conditions after reconstitution.
Gene Name : Amica1 adhesion molecule,interacts with CXADR antigen 1 [ Mus musculus ]
Official Symbol : Amica1
Synonyms : Amica1; adhesionmolecule, interacts with CXADR antigen 1; AMICA; Crea7; Gm638;junctional adhesion molecule-like; mCrea7; adhesion molecule AMICA; dendriticcell-specific protein CREA7; dendritic-cell specific protein Crea7
Gene ID : 270152
mRNA Refseq : NM_001005421
Protein Refseq : NP_001005421
UniProt ID : Q80UL9
Chromosome Location : 9
Function : cell adhesionmolecule binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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What are the downstream signaling pathways activated by AMICA1 and their functional consequences? 12/19/2022

Activation of AMICA1 triggers downstream signaling pathways, such as Rho GTPases, PI3K/AKT, or MAPK/ERK, which modulate cytoskeletal rearrangement, cell survival, and gene expression, impacting cellular functions.

How does AMICA1 contribute to the formation and maintenance of tissue architecture? 02/10/2022

AMICA1 contributes to the formation and maintenance of tissue architecture by promoting cell adhesion, regulating cell polarity, and facilitating tissue-specific cellular organization.

What is the tissue-specific expression pattern of AMICA1 in different organs? 11/29/2021

AMICA1 exhibits a tissue-specific expression pattern, with higher expression observed in epithelial tissues, neural tissues, and certain organs like the liver and kidney.

Can targeting AMICA1 be a potential therapeutic strategy for diseases involving abnormal cell adhesion? 08/28/2020

Targeting AMICA1 signaling or interaction with specific antibodies or small molecules holds potential as a therapeutic strategy for diseases associated with abnormal cell adhesion or tissue integrity.

How does AMICA1 interact with other cell adhesion molecules and extracellular matrix components? 08/26/2020

AMICA1 interacts with various cell adhesion molecules, such as cadherins, integrins, or selectins, as well as extracellular matrix components like collagen or fibronectin, facilitating cell-cell and cell-matrix interactions.

Does AMICA1 have post-translational modifications that impact its function or stability? 02/22/2020

AMICA1 can undergo post-translational modifications, including phosphorylation, glycosylation, or ubiquitination, which can affect its adhesive properties, intracellular signaling, or protein stability.

How does AMICA1 expression change during development and tissue regeneration? 12/16/2018

During development and tissue regeneration, AMICA1 expression is dynamically regulated, showing distinct spatiotemporal patterns that coincide with tissue remodeling and organogenesis.

What is the role of AMICA1 in cell-cell adhesion and cell migration processes? 08/19/2018

AMICA1 plays a crucial role in cell-cell adhesion and cell migration processes, promoting the formation of stable intercellular junctions and regulating cellular movement and tissue morphogenesis.

What is the impact of AMICA1 dysregulation on disease progression or pathological conditions? 12/30/2017

Dysregulation of AMICA1 expression or function has been implicated in various diseases, including cancer metastasis, epithelial barrier dysfunction, or neurodevelopmental disorders, underscoring its involvement in disease pathogenesis.

Can modulation of AMICA1 expression or activity influence cellular phenotypes, such as cell proliferation or differentiation? 07/17/2017

Modulation of AMICA1 expression or activity can influence cellular phenotypes, including cell proliferation, differentiation, and cell fate determination, highlighting its role in tissue homeostasis and development.

Customer Reviews (3)

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Reviews
02/26/2020

    Compatibility with complex biological matrices for translational research.

    07/24/2019

      Enhanced assay sensitivity for detection of subtle protein changes.

      06/30/2018

        Accurate determination of protein function and activity.

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